Nucleic acid amplification method

Abstract

The invention provides a DNA polymerase using oligonucleotide primer and having strand displacement ability, which is capable of amplifying nucleic acid under substantially constant temperature condition. The invention provides a method of amplifying nucleic acid, comprising: under the substantially constant temperature condition, incubating reaction solution comprising at least a deoxy nucleoside triphosphate, at least a DNA polymerase having strand displacement ability, bivalent catio, at least over 0.01% surfactant, at least two oligonucleotide primers and as nucleic acid segment of templet; the polymerase reaction is carried out by taking 3'-back end of the primer as the start, thereby amplifying the nucleic acid segment.

Description

technical field

[0001] The invention relates to a nucleic acid amplification method. More specifically, the present invention relates to a nucleic acid amplification method characterized in that the polymerase reaction is carried out by incubating the reaction solution at a substantially constant temperature using a DNA polymerase having strand displacement ability. Background technique

[0002] In the research of molecular biology, generally speaking, amplification of nucleic acid is carried out by enzymatic methods using DNA polymerase. As a nucleic acid amplification method, polymerase chain reaction (PCR) is a well-known method. In order to amplify the target nucleic acid sequence, the PCR method consists of the following three steps: a step of denaturing double-stranded DNA as a template into single-stranded DNA (denaturation step); and a step of annealing primers to single-stranded DNA (annealing step); and, a step of extending the complementary strand starting from ...

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