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DNA dependent protein kinase catalytic subunit single-chain antibody and its encoding gene and application

A kinase catalytic subunit and single-chain antibody technology, applied in recombinant DNA technology, DNA/RNA fragments, applications, etc., can solve problems such as immune response, liver toxicity, and limit practical applications, and achieve the effect of enhancing radiation sensitivity

Inactive Publication Date: 2009-02-11
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these inhibitors have poor specificity or can cause liver toxicity or immune response when used in the body, which limits their practical application.

Method used

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  • DNA dependent protein kinase catalytic subunit single-chain antibody and its encoding gene and application
  • DNA dependent protein kinase catalytic subunit single-chain antibody and its encoding gene and application
  • DNA dependent protein kinase catalytic subunit single-chain antibody and its encoding gene and application

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Embodiment 1

[0047] Embodiment 1, the cloning of the gene of anti-DNA-PKcs single-chain antibody (anti-DPK3-scFv) and its function

[0048] 1. Antigenicity analysis of catalytic subunit of DNA-dependent protein kinase and expression and purification of corresponding protein fragment (DPK3)

[0049] Analyze the functional domains contained in the DNA-PKcs protein through the NCBI database:

[0050] Including leucine zipper motif (AA1503-1531), autophosphorylation complex (AA2609-2647), caspase-3 action site, FAT domain (AA3023-3472), FATC domain (AA4096-4128), Ku binding region ( AA3002-3580) and kinase active domain (PI3K / PI4K domain) (AA3748-4128).

[0051] The antigenicity of DNA-PKcs was analyzed by Biosun software, and the protein fragment of AA1960-2227 (DPK3) was preliminarily screened out. The amino acid sequence of DPK3 protein is 250 amino acids long, the molecular weight is 29KD, and the isoelectric point is 5.4. DPK3 was located between the leucine zipper motif and the autoph...

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Abstract

The invention discloses a DNA-dependent protein kinase catalytic subunit single-chain antibody, a coding gene thereof and an application thereof. The DNA-dependent protein kinase catalytic subunit single-chain antibody is a fusion protein obtained by connecting polypeptides which are in 1st to 109th counted from the end of amino and of which the amino acid sequence is Sequence 2 in a sequence table with polypeptides which are in 135th to 250th counted from the end of amino and of which the amino acid sequence is Sequence 2 in the sequence table by a connecting peptide. Specifically, the single-chain antibody can be protein of which amino acid sequence is Sequence 2 in a sequence table and the coding gene can be the gene of which nucleotide sequence is Sequence 1 in the sequence table. The single-chain antibody and the coding gene thereof, provided by the invention, can be used for preparing drugs for the treatment of tumors.

Description

technical field [0001] The invention relates to a DNA-dependent protein kinase catalytic subunit single-chain antibody and its coding gene and application. Background technique [0002] Malignant tumors seriously threaten human health. Radiation therapy is one of the main treatment methods for malignant tumors. However, radiation therapy can cause damage to normal tissue surrounding the tumor, leading to serious side effects. At the same time, some tumor cells have biological characteristics of radiation resistance or tumor cells induce resistance during radiotherapy, resulting in reduced therapeutic effect. At present, how to reduce the dose of radiotherapy, improve the therapeutic effect and reduce the tolerance of tumor cells, that is, how to enhance the radiosensitivity of tumor radiotherapy has become one of the hot spots in the field of tumor research. [0003] The killing effect of radiotherapy on tumor cells is mainly through the induction of DNA double-strand bre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/40C12N15/13C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A61K39/395A61K48/00A61P35/00
Inventor 周平坤杜丽周丽君
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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