Bacillus tubercle gene vaccine for treating medicine-tolerant pulmonary tuberculosis
A technology of Mycobacterium tuberculosis and genetic vaccines, applied in the direction of antibacterial drugs, bacterial antigen components, etc.
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Embodiment 1
[0130] It is used to prepare the Mycobacterium tuberculosis gene vaccine for treating drug-resistant tuberculosis, one of which is that the Mycobacterium tuberculosis Ag85A coding gene sequence is cloned into the eukaryotic expression vector pVAX1.
[0131] (1) Gene sequence:
[0132] The gene sequence of the Mycobacterium tuberculosis Ag85A nucleic acid vaccine for the treatment of drug-resistant tuberculosis is as follows:
[0133] 1T TTTCCCGGC CGGGCTTGCC GG TGGAGTAC CTGCAGGTGC CGTCGCCGTC GATGGGCCGT
[0134] 61 GACATCAAGG TCCAATTCCA AAGTGGTGGT GCCAACTCGC CCGCCCTGTA CCTGCTCGAC
[0135] 121 GGCCTGCGCG CGCAGGACGA CTTCAGCGGC TGGGACATCA ACACCCCGGC GTTCGAGTGG
[0136] 182 TACGACCAGT CGGGCCTGTC GGTGGTCATG CCGGTGGGTG GCCAGTCAAG CTTCTACTCC
[0137] 242 ACTGGTACC AGCCCGCCTG CGGCAAGGCC GGTTGCCAGA CTTACAAGTG GGAGACCTTC
[0138]301 CTGACCAGCG AGCTGCCGGG GTGGCTGCAG GCCAACAGGC ACGTCAAGCC CACCGGAAGC
[0139] 361 GCCGTCGTCG GTCTTTCGAT GGCTGCTTCT TCGGCGCTGA CGCTGGCGAT CTATCACCCC
[014...
Embodiment 2
[0189]The tuberculosis gene vaccine used for preparing drug-resistant tuberculosis is cloned into the eukaryotic expression vector pVAX1 to form the ESAT6-Ag85A chimeric gene vaccine.
[0190] (1) Gene sequence:
[0191] The gene sequence of the Mycobacterium tuberculosis Ag85A nucleic acid vaccine for the treatment of drug-resistant tuberculosis is as follows:
[0192] 1T TTTCCCGGC CGGGCTTGCC GG TGGAGTAC CTGCAGGTGC CGTCGCCGTC GATGGGCCGT
[0193] 61 GACATCAAGG TCCAATTCCA AAGTGGTGGT GCCAACTCGC CCGCCCTGTA CCTGCTCGAC
[0194] 121 GGCCTGCGCG CGCAGGACGA CTTCAGCGGC TGGGACATCA ACACCCCGGC GTTCGAGTGG
[0195] 183 TACGACCAGT CGGGCCTGTC GGTGGTCATG CCGGTGGGTG GCCAGTCAAG CTTCTACTCC
[0196] 243 ACTGGTACC AGCCCGCCTG CGGCAAGGCC GGTTGCCAGA CTTACAAGTG GGAGACCTTC
[0197] 301 CTGACCAGCG AGCTGCCGGG GTGGCTGCAG GCCAACAGGC ACGTCAAGCC CACCGGAAGC
[0198] 361 GCCGTCGTCG GTCTTTCGAT GGCTGCTTCT TCGGCGCTGA CGCTGGCGAT CTATCACCCC
[0199] 421 CAGCAGTTCG TCTACGCGGG AGCGATGTCG GGCCTGTTGG ACCCCTCCCA GG...
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