Method for preparing ethanol from raw material containing cellulose
A technology of cellulose and raw materials, applied in the field of ethanol preparation, can solve the problems of low ethanol yield and achieve the effect of improving sugar conversion rate and yield
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Embodiment 1
[0061] This example is used to illustrate the method of the present invention for preparing ethanol using cellulose-containing raw materials.
[0062] (1) Steam explosion
[0063] Test like figure 1 For the corn stalk raw material shown, the water content of the corn stalk is 20% by weight measured according to the above method. The raw material is cut into small sections of 1.5 cm × 0.2 cm × 0.5 cm, and a vertical macerator (Shandong Wenrui Machinery Co., Ltd. ) Is mixed with water whose weight is one third of the weight of the corn stalk raw material and soaked to obtain corn stalks with a water content of 40% by weight. Take 1000 grams of corn stalks with a moisture content of 40% by weight and maintain them at a pressure of 1.8 MPa at 195°C for 5 minutes, and then release the pressure to complete the steam explosion. The resulting steam explosion bankruptcy was stirred and mixed with water at 70°C at a weight ratio of 1:3 for 30 minutes, and then solid-liquid with a LW400 deca...
Embodiment 2
[0082] This example is used to illustrate the method of the present invention for preparing ethanol using cellulose-containing raw materials.
[0083] (1) Steam explosion
[0084] Take 1000 grams of corn stalks with a moisture content of 50% by weight, cut them into 1.3 cm × 0.4 cm × 0.8 cm sections, maintain at 190°C at a pressure of 1.7 MPa for 4 minutes, then release the pressure and complete the steam explosion. 1250 grams of steam explosion product (60% by weight water content).
[0085] According to the method for HPLC analysis of sulfuric acid hydrolyzed steam explosion products described in Example 1, it was determined that 1,250 g of steam explosion products contained 213 grams of cellulose and 63 grams of hemicellulose.
[0086] (2) Enzymatic hydrolysis
[0087] Enzymatically hydrolyze the above-mentioned unwashed steam explosion product according to the method of Example 1. The difference is that after adding water to the enzymolysis tank and stirring, the steam explosion pr...
Embodiment 3
[0090] This example is used to illustrate the method of the present invention for preparing ethanol using cellulose-containing raw materials.
[0091] (1) Steam explosion
[0092] Take 1000 grams of corn stalks with a moisture content of 40% by weight, cut them into 1.2 cm × 0.4 cm × 0.7 cm sections, maintain the pressure at 1.7 MPa at 200° C. for 6 minutes, and then release the pressure to complete steam explosion. The resulting steam explosion bankruptcy was mixed with water at 75°C in a weight ratio of 1:2.5 for 50 minutes, and then solidified with a centrifugal pump LW400 decanter centrifuge (Zhangjiagang Huada Centrifuge Manufacturing Co., Ltd.) at 900 rpm After liquid separation, a total of 2000 grams of solid steam explosion product (water content of 70% by weight) was obtained.
[0093] According to the method of HPLC analysis of sulfuric acid hydrolyzed steam explosion product described in Example 1, it was determined that 2000 g of steam explosion product contained 260 gra...
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