Method for fermentation producing glossy ganoderma polyoses using cyclic packed bed reactor

Abstract

The invention relates to a method for producing lucidum polysaccharide by fermentation through a circulating packed bed reactor, wherein a glossy ganoderma cell is fixed on a cell immobilization vector; a fermentation broth is made to be in the flow state through a circulating pipe, a runner pipe and an atomized spray head; the immobilized cell is fermented; and one round of fermentation is finished, partial fermentation broth is reserved and mixed with a novel culture medium for circulating fermentation. The cell undergoes submerged liquid fermentation in the immobilized state, thereby increasing the fermented cell density and the toxicity resistance and simultaneously increasing the yield and the content of cell secretion; and the immobilized cell can be reused. One part of the fermentation broth outputted is continuously used for product development and the other part of the fermentation broth is mixed with the cell culture medium which is inputted into the reactor and then inputted into the reactor at a constant speed, thereby not only being capable of continuously producing products but also improving the concentration of the products in the fermentation broth.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for producing ganoderma lucidum polysaccharide by fermentation in a circulating packed bed reactor. Background technique [0002] Ganoderma lucidum (Leyss ex Fr.) Krast.) belongs to Basidiomycetes, Polyporaceae, Ganoderma higher fungi. Traditional Chinese medicine believes that Ganoderma lucidum is sweet in taste, warm in nature, non-toxic, and can replenish the five swollen qi of the heart, liver, lung, spleen, and kidney. Modern medical research also shows that the pharmacological components of Ganoderma lucidum are very rich, and the active ingredients include polysaccharides (namely: Ganoderma lucidum polysaccharides), triterpenoids (mainly Ganoderma acids), Ganoderma lucidum polypeptides, 16 kinds of amino acids (including seven kinds of human essential amino acids), proteins, steroids, mannitol, coumarin, alkaloids, organic acids (mainly containing fumaric ac...

AI Technical Summary

Problems solved by technology

However, liquid submerged fermentation uses free cells to produce ganoderma sugar and ganoderma acid. The cell density is low, and the product content and yield are low. This is the bottleneck of the ganoderma polysaccharide and ganoderma acid production industry.

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