Putuo hornbeam embryo culture medium and tissue culture rapid propagation method

A technology of Putuo hornbeam and culture medium, which is applied to the seed embryo medium of Putuo hornbeam and the field of tissue culture rapid propagation, can solve the problems of slow speed and can not meet the requirements, and achieves the effect of making up for poor germination.

Inactive Publication Date: 2009-04-08
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

At present, after many years of seedling and afforestation experiments, 379 seedlings have been cultivated by sowing, cutting, grafting, etc., but the speed is too slow to meet the urgent needs of rapid recovery of this rare species. Exploring and trying to expand the tree by tissue culture and rapid pro

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  • Putuo hornbeam embryo culture medium and tissue culture rapid propagation method

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Example Embodiment

[0013] The present invention is illustrated below in conjunction with the examples: WPM salts and MT vitamins are commercially available, and are formulated according to the conventional method shown in the product specification, and are commonly used in the industry. The appropriate amount of BA is 0.001-0.1mg / L, the optimal amount is 0.01mg / L; the appropriate amount of inositol is 50-150mg / L, and the optimal amount is 100mg / L; the appropriate amount of crystal agar is added to the medium The total weight is 0.2%-0.4%, the optimal amount is 0.25%, the appropriate amount of sucrose added is 1.5%-4.5% of the total weight of the medium, and the optimal amount is 3%; the pH value of the medium is 5.0-6.0, and the optimal value is 5.7 . The list of Examples 1-7 is shown below:

[0014]

[0015] The following uses the best solution of Example 1 (the corresponding raw materials and ratio values ​​of Example 1 in the table of comparison) to illustrate the present invention step by step...

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Abstract

The invention relates to a culture medium for carpinus putoensis embryos, which comprises the following raw materials: a WPM salt, an MT vitamin, a BA (benzyl adenine) which is 0.001mg/l to 0.1mg/l, an inose which is 50mg/l to 150mf/l, a gelrite which occupies 0.2 percent to 0.4 percent of the total weight of the culture medium and a cane sugar which occupies 1.5 percent to 4.5 percent of the total weight of the culture medium. The pH value of the culture medium is 5.0 to 6.0. The process for tissue-culture and rapid reproduction by using the culture medium comprises the following four steps of: the collection and selection of explants, the cleaning and sterilization of seeds and the adoption of emryos, the inoculation and culture, the subculture and root induction and the culture of transplants. The invention can obtain carpinus putoensis regrowths in large volumes by the method of tissue culture and rapid reproduction, thereby saving the unique germplasm resource of the earth. The invention also discloses a method for an excellent culture medium and rapid reproduction of seedlings to the society and lays a foundation for further research of carpinus putoensis and exploration of a new biological engineering.

Description

【Technical Field】 [0001] The invention relates to a culture medium for biological tissues and a method for rapid propagation of tissue culture. 【Background technique】 [0002] Putuo hornbeam (Carpinus putoensis), belongs to the Carpinus genus CarpinusL, Betulaceae. Putuo Carpinus is a rare tree species unique to our country. Its distribution range is extremely narrow. It is only found in the Zhoushan Islands in Zhejiang Province. It is one of the ten nearly extinct tree species in my country. Due to the poor germination of the tree and the short pollen life span, the pollen vitality will gradually abort within seven days and the seed germination rate will be low. It is endangered due to ecological and man-made damage. According to the survey data, Putuo hornbeam in the wild is now only one surviving plant in Putuo Mountain. In view of this, the species protection of Putuo Carpinus has attracted the attention of botany research institutions. At present, after years of breeding and...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 黄丽春俞慈英张翠萍李修鹏林新春
Owner ZHEJIANG FORESTRY UNIVERSITY
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