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Method for improving volume of production of griseoviridin with accelerant addition

A technique of griseogreenin and an accelerator, which is applied in the field of increasing the yield of griseogreenin A and increasing the yield of griseocyanin A, and can solve the problems of low fermentative yield of griseogreenin A and the like

Inactive Publication Date: 2009-04-08
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect of low yield of Giseoviridin A fermentation, and provide a method for improving Giseoviridin A yield by adding a promotor

Method used

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  • Method for improving volume of production of griseoviridin with accelerant addition
  • Method for improving volume of production of griseoviridin with accelerant addition
  • Method for improving volume of production of griseoviridin with accelerant addition

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1 Effects of Simvastatin on Giseoviridin A Synthesis and Bacterial Growth

[0044] like figure 1 As shown, by adding simvastatin of 0.15mM, 0.3mM, 0.45mM final concentration, investigate its influence on the synthesis of griseoviridin A and the growth of Aspergillus cinerea. It was found that simvastatin with a final concentration of 3mM had the greatest promoting effect on the biosynthesis of griseoviridin A, and its production could reach 20.8mg / L, which was 63% higher than that of the control group without any additives. The addition of this substance had almost no effect on the growth of Aspergillus aeruginosa HB1-19. According to the principle of a small number of times and the actual experiment, the best way to add simvastatin is to add 0.1mM simvastatin every 24h, add 3 times (that is, add 3 days continuously), the final concentration is 0.3mM, and the initial addition time is 30h.

Embodiment 2

[0045] Example 2 Effects of phthalic acid on the synthesis of griseoviridin A and the growth of bacteria

[0046] like figure 2 As shown, adding phthalic acid with different final concentrations of 6mM, 12mM and 24mM, the yield of griseoviridin A was 1.12 times, 1.59 times and 1.55 times that of the control (without the addition), respectively. Among them, 12mM phthalic acid can make the yield of griseovirmycin A reach 20.2mg / L, which is 59% higher than that of the control group without any addition. The addition of phthalic acid had no effect on the growth of Aspergillus aeruginosa HB1-19. According to the principle of small amount and many times and actual experiments, the inventors determined that the best way to add phthalic acid is to add 4mM phthalic acid every 24h, add 3 times continuously, the final concentration is 12mM, and the initial adding time is 30h.

Embodiment 3

[0047] Example 3 Effect of adding citric acid on the synthesis of griseoviridin A and the growth of bacteria

[0048] like image 3 As shown, the promoting effect of adding citric acid is more obvious, wherein adding 3 times of citric acid with a final concentration of 12mM can make the output of griseovirmycin A increase to 2.79 times that of the control (without any additive), and the output reaches 35.5 mg / L. The addition of citric acid had no effect on the growth of A. griseus HB1-19. According to the principle of small amount and many times and actual experiments, the best way to add citric acid is to add 4mM citric acid every 24h, add 3 times in a row, the final concentration is 12mM, and the initial addition time is from the fermentation of Aspergillus aeruginosa HB1-19 to 30h .

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Abstract

A method for improving the yield of viridogrisein A by adding a promoter belongs to the technology field of the metabolism regulation optimized fermentation process. The method utilizes marine filamentous fungus Aspergillus glaucus HB1-19 of CCTCC M 206022 as a production strain, simvastatin, phthalic acid, citric acid or short-chain fatty acid, such as acetic acid, propionic acid, malonic acid are independently added in a fermentation culture medium as the promoter, or the promoters are mixedly added for promoting the synthesis efficiency for producing the viridogrisein A. The final yield can achieve 36.2mg / l which is 2.84 times of the original level. The method has important significance for researching the pharmacology and pharmacodynamics of the viridogrisein A.

Description

technical field [0001] The invention relates to a method for increasing the yield of griseovirmycin A, in particular to a method for increasing the yield of griseoviridin A by adding accelerators alone or in combination. It belongs to the technical field of metabolic regulation and optimization of fermentation process. Background technique [0002] Giseoviridin A is a kind of independently isolated marine fungus Aspergillus glaucus (Aspergillus glaucus) HB1-19 (preservation date 2006.3.20, China Center for Type Culture Collection, preservation number CCTCCM 206022) by Ocean University of China. Anthrone lactone compound with intellectual property rights and brand-new structure with anticancer activity. Giseoviridin A uses 9-anthrone as the core, and C-10 and C-4 in the molecule form a six-membered lactone ring. As evaluated by the Shanghai Institute of Materia Medica, Chinese Academy of Sciences, it has a strong inhibitory effect on the proliferation of human lung cancer A5...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C12R1/66
Inventor 周祥山张元兴孙学谦陶可敬蔡孟浩
Owner EAST CHINA UNIV OF SCI & TECH
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