Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for determining homocysteine and homocysteine diagnosis / determination reagent kit

A technology of homocysteine ​​and kits, applied in the field of homocysteine ​​diagnosis/measurement methods and kits, which can solve problems such as poor sensitivity, less use, and narrow linear range

Inactive Publication Date: 2011-03-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 3. Ion chromatography is mainly used in experimental research, and it is rarely used clinically
However, these methods still have some disadvantages, such as narrow linear range, poor sensitivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining homocysteine and homocysteine diagnosis / determination reagent kit
  • Method for determining homocysteine and homocysteine diagnosis / determination reagent kit
  • Method for determining homocysteine and homocysteine diagnosis / determination reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0141] Example 1: Determination of Homocysteine ​​Content in Plasma

[0142] 1) Preparation of standard samples

[0143] Dissolve a certain amount of homocysteine ​​in water or buffer, then adjust the concentration of homocysteine ​​to 200 micromol / L, and mix the obtained solution with the same volume of 3mmol / L 2-mercaptoethanol as a standard sample;

[0144] 2) Pretreatment of samples to be tested

[0145] The plasma sample was mixed with the same volume of 3mmol / L 2-mercaptoethanol as the sample to be tested;

[0146] 3) Blank sample

[0147] The water or buffer solution is mixed with the same volume of 3mmol / L 2-mercaptoethanol as a blank sample, and its homocysteine ​​concentration is 0 g / L;

[0148] The mixing treatment is that the mixture is allowed to stand at room temperature for 10 minutes after mixing with 2-mercaptoethanol.

[0149] B. Preparation of reagent solution:

[0150] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single-dos...

Embodiment 2

[0171] Example 2: Determination of Homocysteine ​​Content in Plasma

[0172] 1) Preparation of standard samples

[0173] Dissolve a certain amount of homocysteine ​​in water, and then adjust the concentration of homocysteine ​​to 200 micromole / liter as a standard sample;

[0174] 2) Preparation of samples to be tested

[0175] Plasma samples are used as samples to be tested;

[0176] 3) Blank sample

[0177] Described water is used as blank sample, and its homocysteine ​​concentration is 0 g / liter;

[0178] B. Preparation of reagent solution:

[0179] Homocysteine ​​Diagnostic / Assay Reagent is a three-dose reagent containing:

[0180] Reagent 1 with the following composition:

[0181] Dithiothreitol 3mmol / L

[0182] Reagent 2 with the following composition:

[0183] Phosphate buffer 100mmol / L

[0184] NADH0.25mmol / L

[0185] Ferricytochrome c 5mmol / L

[0186] Calcium chloride 5mmol / L

[0187] Reagent 3 with the following composition:

[0188] Phosphate buffer 100m...

Embodiment 3

[0210] Example 3: Determination of Homocysteine ​​Content in Plasma Samples

[0211] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:

[0212] B. Preparation of reagent solution:

[0213] Homocysteine ​​Diagnostic / Assay Reagent is a four-dose reagent containing:

[0214] Reagent 1 with the following composition:

[0215] 2-Mercaptoethanol 3mmol / L

[0216] Reagent 2 with the following composition:

[0217] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0218] NADH0.25mmol / L

[0219] Ferricytochrome c 6mmol / L

[0220] Calcium chloride 5mmol / L

[0221] Reagent 3 with the following composition:

[0222] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0223] Ammonium sulfate 1mol / L

[0224] Nitrite reductase (EC 1.7.2.2) 48000U / L

[0225] Nitrite reductase (EC 1.7.1.4) 60000U / L

[0226] Reagent 4 with the following composition:

[0227] Tris(carboxymethyl)aminomethane-hydrochloric acid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention relates to a determination method for homocysteine contents, which uses an indirect enzymatic recycling method, an enzymic colorimetric, and enzyme united technique. The invention also relates to a homocysteine diagnosis / determination kit. The determination method has high sensitivity, small error; therefore, the determination method and the kit can be widely applied to the clinicalexamination.

Description

【Technical field】 [0001] The invention relates to the technical field of medical testing and determination, more specifically, the invention relates to a homocysteine ​​diagnosis / measurement method and a kit thereof. 【Background technique】 [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analysis assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry , Fluorescence polarization immunoassay (FPIA) method, electrochemical method and enzymatic method. [0003] 1. High-performance liquid chromatography (HPLC) is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. It is gradually replaced by enzyme-linked immunoassay in clinical application , Fluorescence polarization analysis and enzymatic method. High-performance liquid chromatography first uses a reducing...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD