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Ochrobactrum CTS-325 and culture method thereof and application thereof in reduction of hexavalent chromium

A technology of CTS-325 and Pallidum, which is applied in the direction of bacteria, can solve the problems of increasing operating costs, process operability, and difficult gas control

Active Publication Date: 2012-08-29
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Li Defu et al. conducted research on sulfate-reducing bacteria (SRB), first put SO 4 2- reduced to H 2 S, then by H 2 S reduces hexavalent chromium and applies it to the treatment of electroplating wastewater, but the treatment process will produce toxic H 2 S gas is difficult to control, thus limiting the application of this method
Japanese scholars once reported that Enterobacter cloacae HO1 (E.cloacae HO1) was used to treat chromium-containing industrial wastewater under anaerobic conditions. In order to form anaerobic conditions in this method, a large amount of nitrogen must be filled in the medium to remove the medium. dissolved oxygen, which greatly increases operating costs and process operability

Method used

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  • Ochrobactrum CTS-325 and culture method thereof and application thereof in reduction of hexavalent chromium
  • Ochrobactrum CTS-325 and culture method thereof and application thereof in reduction of hexavalent chromium
  • Ochrobactrum CTS-325 and culture method thereof and application thereof in reduction of hexavalent chromium

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Example 1. Growth and reduction of hexavalent chromium in four culture media of Pallidum pallidus CTS-325

[0025] 1.1 Recovery of strains

[0026]Take the seed bacteria preserved in liquid nitrogen, insert them into a 100ml Erlenmeyer flask containing 20ml 1# liquid medium (pH 7-8) at a ratio of 1:100, and place them in aerobic, normal pressure, and 30°C conditions. Shake culture overnight in a shaker at 200 rpm to revive the strain.

[0027] 1.2 Cultivation of Paleobacterium pallidus CTS-325 in different media and reduction of hexavalent chromium

[0028] Transfer the resuscitated strains into four 250ml Erlenmeyer flasks containing 100ml of culture medium at a ratio of 1:100 of the bacteria liquid to the culture medium. The composition of the culture medium in the four Erlenmeyer flasks is different. , 1# culture medium (L -1 ): tryptone 10g, yeast extract 5g, sodium chloride 10g pH=7.0~7.5; 2# culture medium (L -1 ): tryptone 10g, beef extract 3g, sodium chlorid...

example 2

[0029] Example 2. Situation of reduction of hexavalent chromium in 1# culture medium by Paleobacterium pallidus CTS-325

[0030] According to Example 1, we selected the 1# medium with the best growth and reduction effect to carry out the following experiments. Different initial concentrations of hexavalent chromium were added to the medium, which were 100mg / L and 200mg / L respectively.

[0031] 2.1 Recovery of strains

[0032] Take the seed bacteria preserved in liquid nitrogen, insert it into a 100ml Erlenmeyer flask equipped with 20ml 1# liquid medium (pH 7-8) at a ratio of 1:100, and place it in aerobic, normal pressure, and 30°C conditions. Shake culture overnight in a shaker at 200 rpm to revive the strain.

[0033] 2.2 Reduction of hexavalent chromium by Paleobacterium pallidus CTS-325 at different initial chromium concentrations

[0034] Transfer the resuscitated strains into two 250ml Erlenmeyer flasks containing 100ml 1# medium according to the ratio of bacterial sol...

example 3

[0037] Example 3. The situation of reducing hexavalent chromium in the optimized 1# medium of Paleobacterium pallidus CTS-325

[0038] In order to solve the problem that Paleobacterium pallidum CTS-325 cannot completely reduce hexavalent chromium in 1# medium, we optimized 1# medium. By adding xylose or sucrose to the 1# medium, or the bagasse left over from the production of xylose and sucrose, it was found that the effect of reducing hexavalent chromium in the optimized 1# medium of Pallidum bacillus CTS-325 was significantly improved, and Hexavalent chromium can be completely reduced when the initial hexavalent chromium concentration is 100mg / L and 200mg / L respectively.

[0039] 3.1 Recovery of strains

[0040] Take the seed bacteria preserved in liquid nitrogen, insert them into a 100ml Erlenmeyer flask containing 20ml 1# liquid medium (pH 7-8) at a ratio of 1:100, and place them in aerobic, normal pressure, and 30°C conditions. Shake culture overnight in a shaker at 200...

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Abstract

The invention discloses Ochrobactrum CTS-325 and a culturing method thereof as well as application to reducing hexavalent chromium, and relates to microbe and application thereof to efficiently and thoroughly reducing the hexavalent chromium under the aerobic condition as well as a culturing method thereof. The Ochrobactrum CTS-325 has a preserving number CGMCC1715. The strain can efficiently andthoroughly reduce the hexavalent chromium. The pH value is between 7 and 9 during reduction of the hexavalent chromium by the strain. The strain comprises one or combination of any few kinds of xylose, ribose, glucose, levulose, galactose, sucrose, maltose, starch, glycerin or mannite in an available culture medium on microbiology. When industrial waste water containing chromium and chromium slag leach liquor are treated by the Ochrobactrum CTS-325 in an optimized culture medium, the hexavalent chromium in the industrial waste water and the chromium slag leach liquor can be reduced to reach sewage discharge standard.

Description

technical field [0001] The invention relates to microorganisms and their application and cultivation method for efficient and thorough reduction of hexavalent chromium under aerobic conditions. Background technique [0002] Chromium salt products are indispensable and important chemical raw materials for the development of the national economy, and are widely used in electroplating, printing and dyeing, leather tanning, wood preservation, and oil exploration. In the process of production and application of chromium salt products, a large amount of chromium pollution will inevitably be produced, which seriously threatens human health. According to reports, drinking water contaminated by chromium can cause abdominal discomfort and diarrhea and other poisoning symptoms; chromium is a skin allergen, which can cause allergic dermatitis or eczema; chromium can stimulate and corrode the respiratory tract, causing rhinitis, Pharyngitis, bronchitis, severe nasal septum erosion, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02
Inventor 林璋马晓艳程扬健栗斌廖逸群吴兆贤
Owner FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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