Unlock instant, AI-driven research and patent intelligence for your innovation.

Separation process for plant endophyte

A technology of plant endophytes and endophytes, applied in the field of microbiology, can solve the problem of endophytes not being separated and achieve good separation effect

Inactive Publication Date: 2010-09-15
HUNAN UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during centrifugation, many endophytes may still be present in the plant tissue without being isolated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Separation process for plant endophyte
  • Separation process for plant endophyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Two methods were used to separate the endophytes in the four plants of Nightshade Solanum, Dendrobium, Lemon 7, and Water Chicken; figure 1 Shown; Adopt the method of the present invention to separate endophyte flow process such as figure 2 shown.

[0027] Subsequently, the plates obtained by the above two methods were cultured at a constant temperature of 30°C for 2 to 14 days, and then different forms of colonies were picked, fungi were transferred to Chapeauer medium, bacteria were transferred to TSB solid medium, cultured by streaking until pure, and counted The results are shown in the table below:

[0028]

[0029]

[0030]

[0031]

[0032] It can be seen from the results that the total number of four kinds of endophytic bacteria isolated by the method of the present invention is obviously 3 to 5 times more than the total number of endophytic bacteria obtained by the method of the present invention; Bacteria. The method...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for obtaining plant endophytes by separation, and the method comprises the steps of plant pre-treatment, surface disinfection, leaching of the endophytes, separation and purification of the endophytes, and the like; compared with the ordinary method, the method not only utilizes mechanical grinding and shearing force to leach the plant endophytes, but also adds a surfactant and a cellulose enzyme to decompose plant fibers and plant cell walls, thereby leading the endophytes in plant tissues to be released more fully and obtaining better separation effect of theplant endophytes.

Description

technical field [0001] The invention belongs to the field of microbiology, and relates to a method for comprehensively separating and obtaining endophytic bacteria from plants Background technique [0002] Plant endophytes widely exist in various plants, and they have a huge impact on the life activities of the host. At present, the research on plant endophytes is becoming a hot spot in the fields of biology, environment, and chemistry in the world. The existing published plant endophyte isolation method 200710065724 mainly uses mechanical force grinding and centrifugation to separate plant endophyte from plant tissue homogeneity. However, during centrifugation, many endophytes may still be present in the plant tissue without being isolated. However, the research on plant endophytes is eager to obtain all the endophytes in the plant host as much as possible, and the method used in the present invention can obtain all the endophytes in the plant host to the greatest extent. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00
Inventor 罗胜联肖潇万勇陈亮郭函君曹喆苏峰
Owner HUNAN UNIV