Preparation of dye doped silicon dioxide fluorescent nanoparticle

A silicon dioxide and fluorescent nanotechnology, applied in chemical instruments and methods, luminescent materials, reagents, etc., can solve problems affecting detection results, dye leakage, etc.

Inactive Publication Date: 2009-06-24
SOUTHEAST UNIV
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  • Abstract
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Problems solved by technology

These three methods generally have a common shortcoming, that is, there are large or small pore structures on the surface of the particles, which wi

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  • Preparation of dye doped silicon dioxide fluorescent nanoparticle
  • Preparation of dye doped silicon dioxide fluorescent nanoparticle
  • Preparation of dye doped silicon dioxide fluorescent nanoparticle

Examples

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Embodiment 1

[0019] Add 3.54ml of Triton X-100, 15ml of cyclohexane, 3.6ml of 1-n-hexanol, 0.1ml of an aqueous solution in which 1.8mg of DAA is dissolved in a three-necked flask, and form a water-in-oil inverse microemulsion under stirring. Then 200 μl of VTES monomer and 10 μl of aminopropyltriethoxysilane were added. After reacting at room temperature for 24 hours, add 0.08 g of azobisisobutyronitrile, react at 65° C. for 4 hours under nitrogen, cool, and add ethanol to break the emulsion. The generated nanoparticles were collected by centrifugation, ultrasonically washed twice with acetone, and dried in vacuum at room temperature.

[0020] The average particle diameter of the nanoparticle prepared by this method is 30nm, and the monodispersity is better, and its aqueous dispersion produces strong green fluorescence under the excitation of 400nm excitation light, and the maximum emission wavelength is positioned at 525nm (attached). figure 1 ). After the nanoparticles were dispersed i...

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Abstract

The invention provides a method for preparing dye doping type silicon dioxide fluorescent nanoparticles, characterized by comprising the steps of: selecting an organic fluorescent dye having a double-bond substituent and a silane monomer having double-bond substituent; utilizing the hydrolytic polymerization of the silane monomer to form the nanoparticles with the organic dye as the core and silicon dioxide as the shell; then adding a free radical initiator to a reaction system, initiating the silicon substrate on the surface of the nanoparticles and on the pore path and the double bonds on the dye to copolymerize so that the dye is covalently bonded with the silane to prevent the nanoparticles from leaking in the process of detection. The nanoparticles with the organic dye as the core and silicon dioxide as the shell from the preparation are mono-dispersed spherical and have particle diameter of about 30 nanometers. The dispersion liquid of the nanoparticles in the water generates strong green fluorescence under the excitation of 400nm excitation light; the maximum emitting wavelength of the green fluorescence is 525nm, the fluorescence intensity can remain 92% of the initial fluorescence intensity even if the fluorescence is dispersed in the water solution for 72 hours.

Description

technical field [0001] The invention specifically relates to a method for preparing doped fluorescent nanoparticles with organic dye as the core and silicon dioxide as the shell. The invention belongs to the technical field of luminescent nanometer materials. Background technique [0002] Dye-doped silica fluorescent nanoparticles can be used as nanoprobes for cell staining, immunoassay, drug delivery and DNA tracing, etc., and more and more attention has been paid to the application in the field of life sciences. Since multiple dye molecules can be doped in one nanoparticle, this kind of nanoprobe not only has higher sensitivity than the widely used fluorescent molecular probes such as FITC, but also has the hydrophilicity of silica. . In addition, the dye is separated from the detection solution by the core silica as the shell structure, which can effectively inhibit the drift of the detection solution polarity on the dye emission wavelength and the quenching effect on t...

Claims

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Application Information

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IPC IPC(8): C09K11/06C09K11/02
CPCY02P20/141
Inventor 王明亮董宝利周盛蕊
Owner SOUTHEAST UNIV
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