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Method for establishing rubber tree internal integument regeneration system

A rubber tree and system technology, applied in the field of plant tissue and cell engineering, can solve problems such as the inability to establish an inner bead regeneration system, and achieve the effects of improving experimental and production efficiency, prolonging time, and accelerating development.

Inactive Publication Date: 2009-07-22
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

However, practice has proved that the inner integument regeneration system of domestic varieties cannot be established with this method, and related research is still in the exploratory stage (Cong Ming. Human Serum Albumin cDNA Transformation of Hevea, Master Thesis of South China Tropical Agricultural University, 2005)

Method used

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Embodiment

[0026] A kind of method that establishes rubber tree integument regeneration system, concrete steps are:

[0027] (1) Selection and treatment of young fruit: Select 6-10 week old Reyan 88-13 young fruit, wash it with washing powder first, then rinse it fully with flowing tap water, move it into a sterile environment, and wash it with 75% ethanol first. Disinfect for 1 minute, then disinfect with 0.1% mercury liter for 10 minutes, and finally rinse with sterile water 3 to 5 times, 3 minutes each time.

[0028] (2) Selection and treatment of explants: Cut the sterilized young fruit, take out the ovule, cut off the outer integument of the ovule, keep the inner integument and nucellus, and cut it into a size of 0.3-1.0cm , Thick 0.5 ~ 1mm sheet.

[0029] (3) Induction process of callus: inoculate the above sheet explants in callus induction medium and culture in dark for 15-30 days, the culture temperature is between 24°C-28°C. The explants expanded, partially callused, and the ...

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Abstract

The invention relates to a method for taking rubber tree inner integument as an explant, in particular to a method for establishing a rubber tree inner integument regeneration system. The method comprises the following main steps: the selection and treatment of young fruits, the selection and treatment of the explant, the induction and subculture of calli, the differentiation of body cell embryoids, and the plant regeneration. The method for establishing the rubber tree inner integument regeneration system has the advantages of simple technological process and low production cost. The inner integument is used to obtain a young scion root clone, thereby overcoming the defects of difficult stamen stripping and serious pollution in a powdery mildew prevalence season lying in the anther culture, and enlarging the source of the explant. The method is characterized by easily obtaining the explant and having the robust regenerated plant. The method provides the good regeneration system for the generalization of the young scion root clone and the seed selection of rootstock, provides a good receptor for genetic transformation, and also provides a new source for the embryo calli with long-time subculture. Therefore, the method has wide application prospect.

Description

technical field [0001] The invention relates to a method for regenerating rubber tree plantlets through the somatic embryogenesis pathway as the rubber tree inner integument as an explant, which belongs to the field of plant tissue and cell engineering, in particular to a method for establishing the rubber tree inner integument regeneration system. Background technique [0002] The earliest planting materials used in the production of rubber trees are seedlings, which come from free-pollinated seeds, which have the advantages of fast growth, long economic life, and strong stress resistance, but there are large differences between plants and low average yield (only for budding trees). 1 / 3 of the female parent, can not keep the good traits of the female parent (Wang Zeyun, Chen Xiongting, Wu Hudie. Rubber tree new planting material-somatic embryo plant. Tropical Agricultural Science, 2001, (6): 11-15). The planting material commonly used at present is budding seedlings, and bu...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 黄天带黄华孙李维国李哲孙爱花周权男
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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