Mesenchyma stem cell for inhibiting osteoclast generation, as well as preparation method and application thereof

A stem cell-inhibiting osteoclast technology, applied in biochemical equipment and methods, bone/connective tissue cells, animal cells, etc., can solve the problems of application inhibition and achieve the effect of inhibiting osteoclast resorption

Inactive Publication Date: 2009-07-22
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For a long time, the treatment of related diseases caused by osteoblast activity disorder or osteoclast activity hyperactivity such as femoral head ischemia, femoral head necrosis, bone injury, etc. has mainly relied on drugs, surgical replacement, autologous bone graft and allogeneic bone graft. However, their applications are inhibited by their respective shortcomings.

Method used

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  • Mesenchyma stem cell for inhibiting osteoclast generation, as well as preparation method and application thereof
  • Mesenchyma stem cell for inhibiting osteoclast generation, as well as preparation method and application thereof
  • Mesenchyma stem cell for inhibiting osteoclast generation, as well as preparation method and application thereof

Examples

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Embodiment 1

[0021] Example 1. Preparation of Mesenchymal Stem Cells Inhibiting Osteoclastogenesis

[0022] 1) Isolation and culture of mouse mesenchymal stem cells

[0023] Take 2-week-old healthy C57BL / 6 female mice (provided by the Military Medical Science Animal Center), kill them by neck dislocation, soak them in 75% alcohol for 5 minutes, separate the mouse femur and tibia under aseptic conditions, remove the surface tissue, and use The syringe draws the phosphate buffer saline (sodium chloride 8g / L, disodium hydrogen phosphate 2.9g / L, potassium chloride 0.2g / L, potassium dihydrogen phosphate) containing 10% (volume percentage) fetal bovine serum (Hyclone company) 0.24g / L, the solvent is deionized water), rinse the bone marrow cavity, and then carefully cut the femur and tibia into 2mm 2 The fine bone slices were placed in a solution of 0.15% collagenase type II (Sigma company) at a concentration of 0.15% by weight and digested for 1 hour at 37°C. α-MEM medium with bovine serum. D...

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Abstract

The invention discloses a mesenchymal stem cell that inhibits osteoclast generation, and a preparation method and applications thereof. The preparation method of the mesenchymal stem cell leads the mesenchymal stem cell to be cultivated in a culture medium that contains tumor necrosis factors and consequently obtains the mesenchymal stem cell with inhibition capabilities of osteoclast generation and/or bone absorption. The mesenchymal stem cell can be used for preparing medicines that can inhibit osteoclast generation or bone absorption. The mesenchymal stem cell that inhibits osteoclast generation and the preparation method thereof establish foundation for carrying out tissue engineering research.

Description

technical field [0001] The invention relates to a mesenchymal stem cell inhibiting osteoclast formation, a preparation method and application thereof. Background technique [0002] Mesenchymal stem cells are a kind of adult stem cells with multi-directional differentiation ability, which can differentiate into osteoblasts, chondrocytes, adipocytes, tendon tissue, neurons, endothelial cells, liver cells and other tissue cells under suitable conditions . In addition to their multitissue differentiation potential, mesenchymal stem cells have low immunogenicity and the ability to suppress immune responses, allowing allogeneic infusion without triggering rejection. Basic research and clinical research at home and abroad have fully confirmed the effectiveness of mesenchymal stem cells in related treatments. Osteoclasts (OC) are the main cells that absorb bone tissue in humans and animals. Under physiological conditions, osteoclasts and osteoblasts cooperate with each other to pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/08A61K35/32A61K35/28A61K35/12A61P19/08C12N5/0775
Inventor 毛宁朱恒江小霞张毅
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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