Method for screening pseudomonas syringae pv.tabaci antagonistic bacteria
A technology of germs and wildfires, applied in the field of biological control, can solve the problems of needing improvement and low screening efficiency, and achieve the effect of wide adaptability and improved screening efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0024] (1) Cultivation of bacteria to be tested, tobacco wildfire bacteria
[0025] The bacterial strains to be tested are 040239 and 050117, a total of 2 strains, the tobacco wildfire strain pst05, all isolated from tobacco diseased leaves. 5 points of 2 bacterial strains to be tested were spot-connected on NA or KB plates (in this example, 5 points of the same plate were the same strain, but 5 different bacterial strains could be used in specific implementation), tobacco wildfire pst05 was inoculated by streaking, 28 ± 1 Cultivate for 2 to 3 days at ℃;
[0026] (2) Preparation of Tobacco Wildfire Bacteria Suspension
[0027] Take 10-15ml of 0.1% Tween 80 sterilized aqueous solution and add it to the cultivated tobacco wildfire bacteria plate, scrape off the bacterial lawn, transfer it to a sterilized Erlenmeyer flask, and adjust the bacterial concentration with 0.1% Tween 80 sterilized aqueous solution for 10 4~5 cfu / ml, the suspension of tobacco wildfire bacteria can be ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap