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Chromatographic test device

A technique of utensils and ingredients, applied in the field of chromatography test utensils, can solve the problems of misjudgment, unclear marking of measuring parts, and inability to correctly identify markings with background enhancement.

Inactive Publication Date: 2009-09-30
SYSMEX CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, as described in Japanese Patent Publication No. 2000-258418, when using a chromatography test device equipped with a blood cell separation unit and a reagent unit in the order in which the samples pass through, the labeled antibody remains in the developing layer, resulting in a problem of background enhancement.
In the chromatographic test equipment, the determination of the presence or absence of the analyte is realized by capturing the analyte labeled with the marker on the chromatographic carrier, and confirming the mark appearing on the measurement part with the naked eye. Therefore, if the background is enhanced, The marks on the measured parts are difficult to identify and difficult to make judgments
Especially when there are very few objects to be tested, the marks on the measurement parts will not be clear, and the background enhancement will not be able to correctly identify the marks, which may lead to misjudgment

Method used

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Examples

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Embodiment 1

[0123] The constitution of the test equipment of embodiment 1

[0124] name size Types of manufacturers Mark fixed part 2 10mm×5mm 8975 PALL The first - blood cell separation unit 3 20mm×5mm MF whatman The second blood cell separation unit 4 10mm×5mm BTS SP300 PALL Sealing part 9 10mm×5mm 7759 Adhesives Chromatography carrier 5 30mm×5mm SHF 13504 Millipore Substrate 8 100mm 9020 Adhesives Protective film 11 20mm×5mm 7759 Adhesives Protective film 12 40mm×5mm 7759 Adhesives Protective film 13 40mm×5mm 7759 Adhesives Absorbent Part 7 35mm×5mm CF5 whatman

[0125] 2. Production of the test equipment of Comparative Example 1

[0126] Such as figure 2 The test equipment of Comparative Example 1 was produced as shown. The test tool of Comparative Example 1 is different from the test tool of Example 1 in that it is composed of a first blood cell separation uni...

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Abstract

A chromatographic test device comprising, in the order of passage of a sample containing blood cell components and liquid components, a label holding member provided with a labeling substance capable of binding specifically to an analyte contained in the liquid components, a blood cell separation member for separating predetermined blood cell components in the sample from the liquid components, and a chromatographic carrier carrying a capturing substance capable of binding specifically to the analyte.

Description

Technical field: [0001] The present invention relates to a chromatographic test tool for detecting the analyte in a sample by using a substance that can specifically bind to the analyte, and the chromatographic test tool is used for inspection with whole blood. Background technique: [0002] Chromatography test kits are examples of test kits for detecting analyte in a sample using a substance that can specifically bind to the sample. In the past, such chromatographic test kits were mainly used to detect analytes in serum samples, but in recent years, chromatographic test kits for whole blood have appeared. [0003] For example, Patent Publication No. 2000-258418 discloses a chromatographic test device, which has a blood cell separation unit that can remove blood cell components in whole blood and allow only plasma components to pass through. When a sample (whole blood) is added to this test device, only the plasma component in the sample passes through the blood cell separa...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/54386G01N33/558G01N33/54387
Inventor 真锅智子
Owner SYSMEX CORP
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