Method for quickly inducing bulblet by lily bulb scale slice

A technology of small bulbs and scales, which is applied in the field of efficient production of lily bulbs, can solve the problems of low scale cutting propagation efficiency, limited parts of small bulbs, and many small bulb processes, etc., and achieve the goals of increased productivity, reduced process and time, and high-yield production Effect

Inactive Publication Date: 2009-10-14
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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Problems solved by technology

[0004] In view of the low propagation efficiency of scale cuttings in the prior art, the tissue culture rapid propagation technology not only has a low seed rate, but also produces small bulbs with limited parts, mainly callus and adventitious buds, and there are many processes for forming small bulbs. It takes a long time and is prone to problems such as variation. The present invention provides a method for quickly inducing small bulbs by using lily scale slices to quickly and mass-produce lily bulblets with high quality and low cost to meet market demand.

Method used

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  • Method for quickly inducing bulblet by lily bulb scale slice
  • Method for quickly inducing bulblet by lily bulb scale slice

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Embodiment 1

[0019] 1. Low-temperature dormancy breaking treatment for bulbs: After harvesting the bulbs of the Lily Oriental variety "Siberia" and the OT variety "Huangtianba", wash them with water, and wash them with 600 times chlorothalonil + 500 times Daisenzin + 500 times After 20 minutes of immersion treatment with pentachloronitrophenylphosphine, use commercially available peat as a substrate to embed and place in a refrigerator at 4°C, and take out the bulbs after 45 days;

[0020] 2. Scale cleaning and disinfection: peel off the scales of the bulbs layer by layer, and discard the outermost scales of the first and second layers; take the scales after the third layer, rinse them with tap water, and then use an appropriate concentration Wash and rinse the scales in 75% alcohol for 20 seconds, then put them in 0.15% mercury solution, vibrate on a shaker for 25 minutes, and then soak them in 2% alcohol Soak in sodium hypochlorite solution for 20 minutes, and finally rinse with sterile ...

Embodiment 2

[0029] 1. The low-temperature broken dormancy treatment of bulbs: kind and processing method are the same as embodiment 1;

[0030] 2. Scale cleaning and disinfection: the operation is the same as in Example 1. During the disinfection treatment, shake and wash with 0.15% mercuric chloride for 20 minutes, that is, shake on a shaking table with a rotating speed of 200r / min, and then soak in 2% sodium hypochlorite solution for 20 minutes , and finally rinsed 3 times with sterile water;

[0031] 3. Scale pre-cultivation: inoculate the sterilized scales into MS basic medium, and pre-cultivate them for 10 days at a temperature of 28°C and a light intensity of 1500LX;

[0032] 4. Scale section: operation is the same as embodiment 1;

[0033] 5. Slice induction culture: Inoculate the excised scales one by one into the bulb induction medium of MS+NAA0.5mg / L+agar 6~6.5g / L+sugar 70g / L. One side is down, the pH value of the medium is 5.8, and after inoculation, it is placed in a dark in...

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Abstract

The invention provides a method for quickly inducing bulblet by lily bulb slice, which comprises the steps of seed ball low-temperature dormancy breaking treatment and scale disinfection treatment. The method is characterized in that the scale disinfection comprises the following treatments: scale pre-culture, scale slicing, bulblet inducing culture, bulblet culture, treatment before bulblet bottle-out and field setting. Each scale can produce 20 to 30 bulblets, each seed ball can produces more than 300 bulblets, the method not only can produce bulb-let with high yield and high efficiency rapidly, but also can form single bulblet directly and does not have the multi-pistil problem that the bulb-let is induced from adventitious bud; and the bulblet can be formed by direct inducing culture, thus reducing the process and the time for the callus tissues and the adventitious bud which require at least two times of secondary change over to form bulblet in the prior art, and simultaneously avoiding the mutation of the bulblet induced from bulblet. The bulblet induction and culture are performed in darkness, thereby effectively saving the energy source.

Description

technical field [0001] The invention relates to a method for efficiently producing lily bulblets, belonging to the technical field of plant cultivation. Background technique [0002] Lily (Lilium spp.) is a plant of the genus Lilium (Lilium) in the family Liliaceae. It is an important bulbous flower in the world. It has the characteristics of beautiful flower shape, bright color and pleasant fragrance, and is deeply loved by people. However, the bulbs used in my country's current production mainly rely on imports, and 150 million bulbs are needed every year. In recent years, the state and various provinces have supported the localization of lily bulbs, and a small amount of domestic bulbs have been put on the market. According to statistics, there were about 25 million domestic bulbs in 2008, which is less than the annual demand for lily bulbs in my country. There is a considerable gap. Due to the immature bulb breeding technology in our country, and the long breeding cycle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吴丽芳崔光芬张艺萍吴学尉康超勇汪国仙王继华和葵
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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