Method for quickly inducing bulblet by lily bulb scale slice
A technology of small bulbs and scales, which is applied in the field of efficient production of lily bulbs, can solve the problems of low scale cutting propagation efficiency, limited parts of small bulbs, and many small bulb processes, etc., and achieve the goals of increased productivity, reduced process and time, and high-yield production Effect
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Embodiment 1
[0019] 1. Low-temperature dormancy breaking treatment for bulbs: After harvesting the bulbs of the Lily Oriental variety "Siberia" and the OT variety "Huangtianba", wash them with water, and wash them with 600 times chlorothalonil + 500 times Daisenzin + 500 times After 20 minutes of immersion treatment with pentachloronitrophenylphosphine, use commercially available peat as a substrate to embed and place in a refrigerator at 4°C, and take out the bulbs after 45 days;
[0020] 2. Scale cleaning and disinfection: peel off the scales of the bulbs layer by layer, and discard the outermost scales of the first and second layers; take the scales after the third layer, rinse them with tap water, and then use an appropriate concentration Wash and rinse the scales in 75% alcohol for 20 seconds, then put them in 0.15% mercury solution, vibrate on a shaker for 25 minutes, and then soak them in 2% alcohol Soak in sodium hypochlorite solution for 20 minutes, and finally rinse with sterile ...
Embodiment 2
[0029] 1. The low-temperature broken dormancy treatment of bulbs: kind and processing method are the same as embodiment 1;
[0030] 2. Scale cleaning and disinfection: the operation is the same as in Example 1. During the disinfection treatment, shake and wash with 0.15% mercuric chloride for 20 minutes, that is, shake on a shaking table with a rotating speed of 200r / min, and then soak in 2% sodium hypochlorite solution for 20 minutes , and finally rinsed 3 times with sterile water;
[0031] 3. Scale pre-cultivation: inoculate the sterilized scales into MS basic medium, and pre-cultivate them for 10 days at a temperature of 28°C and a light intensity of 1500LX;
[0032] 4. Scale section: operation is the same as embodiment 1;
[0033] 5. Slice induction culture: Inoculate the excised scales one by one into the bulb induction medium of MS+NAA0.5mg / L+agar 6~6.5g / L+sugar 70g / L. One side is down, the pH value of the medium is 5.8, and after inoculation, it is placed in a dark in...
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