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Method for breeding test-tube plantlets of Paphiopedilum henryanum Braem seeds

A cultivation method and test-tube seedling technology are applied in the field of plant tissue cultivation, and can solve the problems of low seed setting rate, difficulty in in vitro cultivation of seeds, difficulty in aseptic sowing and tissue cultivation, etc.

Inactive Publication Date: 2011-11-23
SUBTROPICAL CROPS INST OF GUIZHOU PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the rate of artificial pollination of Paphiopedilum is low, and its aseptic sowing and tissue culture are difficult. It is one of the most difficult genus of seed tube culture in Orchidaceae.

Method used

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Embodiment Construction

[0036] The present invention will be described in detail below in conjunction with specific embodiments.

[0037] (1) Seed germination: first sterilize the P. henley fruit, take the 150-day-pollination P. henley fruit and wash it with tap water, sterilize it with 75% alcohol for 30 seconds, and then sterilize it with 0.1% mercuric chloride solution for 15 minutes. Rinse with bacterial water 5 times, then immerse in 95% alcohol for 1 second, burn off the alcohol on the surface of the fruit after taking it out, cut open the fruit on an ultra-clean workbench, take out the seeds and inoculate them on the seed germination medium, and culture in the dark Change to weak light culture after 3 weeks, until seed develops into protocorm, the composition of seed germination medium is: 1 / 4MS+15% (volume) coconut milk+20g / L sucrose+1g / L gac+0.6% agar, PH 5.2-5.4; Here, the medium with low salt concentration is selected, which is conducive to the germination of seeds, and the germination rat...

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Abstract

The invention discloses a method for breeding test-tube plantlets of Paphiopedilum henryanum Braem seeds, comprising seed bourgeoning, proliferation and differentiation, and sound seedling rootage. A culture medium for the seed bourgeoning includes 1 / 4 of MS, 15 percent (in volume) of coconut milk, 20 g / L of sucrose, 1 g / L of activated carbon and 0.6 percent of agar, with a PH of 5.2-5.4; a culture medium for the proliferation and differentiation comprises 1 / 2 of MS, 0.5-2.0 mg / L of 6-BA, 0.2 mg / L of NAA, 2 g / L of tryptone, 15 percent (V / V) of coconut milk, 20 g / L of sucrose, 1 g / L of activated carbon and 0.6 percent of agar, with a pH of 5.2-5.4; and a culture medium for the sound seedling rootage comprises 1 / 2 of MS, 1.0 mg / L of NAA, 15 percent (in volume) of coconut milk, 20 g / L of sucrose, 1 g / L of activated carbon and 0.6 percent of agar, with a PH of 5.2-5.4. The method achieves over 45 percent of seed bourgeoning rate and over 90 percent of planting rate and transplantation survival rate.

Description

technical field [0001] The invention relates to the technical field of plant tissue cultivation, in particular to an aseptic cultivation method for test-tube seedlings of Paphiopedilum henrifolia. Background technique [0002] Paphiopedilum henryanum Braem (Paphiopedilum henryanum Braem) is a plant of the Orchidaceae (Orchidaceae) Paphiopedium (Paphiopedium), which is a national first-level key protected endangered species. "Washington Convention" ("Convention on International Trade in Endangered Species of Wild Fauna and Flora", Convention on International Trade in Endangered Species of Wild Fauna and Flora, CITES) includes all Paphiopedilum species in its Appendix I list, which is strictly protected by the international community . Paphiopedilum is the most distinctive group of Orchidaceae, and it is also the most peculiar ornamental orchid. The flower shape is peculiar, and the lips are pocket-shaped, which resembles the slippers of old European ladies, so it is also ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 罗晓青
Owner SUBTROPICAL CROPS INST OF GUIZHOU PROVINCE
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