Tissue-culture rapid-propagation culture medium for sea-buckthorn and tissue-culture rapid-propagation method

A technology of tissue culture rapid propagation and culture medium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as easy to spread diseases, low root tiller reproduction coefficient, difficulty in maintaining good varieties, etc.

Inactive Publication Date: 2018-11-02
新疆阿勒泰地区林业工作管理站
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Seabuckthorn is dioecious, and it is difficult to maintain the characteristics of excellent varieties in production by seed reproduction; the reproduction c

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Seabuckthorn tissue culture rapid propagation method of the present invention is:

[0050] (1) Selection and treatment of plant materials: Take the stem section with dormant buds as the test material, first rinse it with tap water, then soak it in detergent solution for 15 minutes, stir continuously during this period, and gently scrub the surface with a soft brush, then use Rinse with tap water, then rinse under running water for 3 hours. Then put it into a sterilized beaker and immediately place it on an ultra-clean workbench, first soak it in 75% alcohol for 30 seconds, then rinse it with sterile water for 3 times, and then soak it in 0.1% mercuric chloride for 4 minutes. Shake continuously during this period, and finally rinse with sterile water 6 times.

[0051] (2) Induction of stem segments with dormant buds: put the stem segments with dormant buds on sterilized filter paper, blot the water dry, cut into 0.8 cm stem segments with single buds and inoculate them o...

Embodiment 2

[0059] Seabuckthorn tissue culture rapid propagation method of the present invention is:

[0060] (1) Selection and treatment of plant materials: Take the stem section with dormant buds as the test material, first rinse it with tap water, then soak it in detergent solution for 10 minutes, stir continuously during this period, and gently scrub the surface with a soft brush, then use Rinse with tap water, then rinse under running water for 2 hours. Then put it into a sterilized beaker and immediately place it on an ultra-clean workbench, first soak it in 75% alcohol for 30 seconds, then rinse it twice with sterile water, and then soak it in 0.1% mercuric chloride for 3 minutes. Shake continuously during this period, and finally rinse with sterile water 5 times.

[0061] (2) Induction of stem segments with dormant buds: put the stem segments with dormant buds on sterilized filter paper to blot the water, cut into 0.5 cm stem segments with single buds and inoculate them on the in...

Embodiment 3

[0069] Seabuckthorn tissue culture rapid propagation method of the present invention is:

[0070] (1) Selection and treatment of plant materials: Take the stem section with dormant buds as the test material, first rinse it with tap water, then soak it in detergent solution for 15 minutes, stir continuously during this period, and gently scrub the surface with a soft brush, then use Rinse with tap water, then rinse under running water for 2.5 hours. Then put it into a sterilized beaker and immediately put it on the ultra-clean workbench, first soak and disinfect with 75% alcohol for 30 seconds, then rinse with sterile water for 3 times, and then immerse and disinfect with 0.1% mercuric chloride for 5 minutes. Shake continuously during this period, and finally rinse with sterile water 6 times.

[0071] (2) Induction of stem segments with dormant buds: put the stem segments with dormant buds on sterilized filter paper to blot the water, cut into 1.1 cm stem segments with single ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a tissue-culture rapid-propagation culture medium for sea-buckthorn. The tissue-culture rapid-propagation culture medium comprises an induced culture medium, a multiplication culture medium and a rooting culture medium, wherein the formula of the induced culture medium contains the following components: 1/2 MS, 30g/L of saccharose, 6g/L of agar, 1.0mg/L-4.0mg/L of 2, 4-D and0.1mg/L-0.4mg/L of NAA; the formula of the multiplication culture medium contains the following components: 1/2 MS, 30g/L of saccharose, 6g/L of agar, 0.5mg/L-3.0mg/L of 6-BA and 0.1mg/L-0.3mg/L of NAA; and the formula of the rooting culture medium contains the following components: 1/2 B5, 30g/L of saccharose, 6g/L of agar and 0.1mg/L-0.4mg/L of NAA. The invention further provides a corresponding tissue-culture rapid-propagation method for the sea-buckthorn. By utilizing the culture medium and the rapid-propagation method, the induction rate of stems with dormant buds is 87%-100%, the multiplication rate is 85%-96%, and the rooting rate is 92%-97%.

Description

technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture technology, and in particular relates to a seabuckthorn tissue culture rapid propagation medium and a tissue culture rapid propagation method. Background technique [0002] Seabuckthorn (Latin scientific name: Hippophae rhamnoides Linn.) is a perennial deciduous fruit tree, shrub or small tree. Its fruit, peel, leaf, bark and its processed products contain more than 280 kinds of physiologically active substances, which have strong special effects of anti-inflammatory, bactericidal, pain-relieving and promoting tissue regeneration. Many of the ingredients have shown miraculous therapeutic effects in killing and inhibiting tumor cells, anti-radiation, anti-coagulation, lowering blood pressure, preventing vascular embolism, anti-aging, anti-fatigue, enhancing body vitality and immunity. [0003] Seabuckthorn has luxuriant branches and leaves, well-developed lateral ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 赵英徐航刘伟郑新国胡茵韩晓燕张志刚
Owner 新疆阿勒泰地区林业工作管理站
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap