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Tissue-culture rapid-propagation culture medium for sea-buckthorn and tissue-culture rapid-propagation method

A technology of tissue culture rapid propagation and culture medium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as easy to spread diseases, low root tiller reproduction coefficient, difficulty in maintaining good varieties, etc.

Inactive Publication Date: 2018-11-02
新疆阿勒泰地区林业工作管理站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Seabuckthorn is dioecious, and it is difficult to maintain the characteristics of excellent varieties in production by seed reproduction; the reproduction coefficient of root tiller reproduction is low
At present, the survival rate of cutting propagation used in production is not high, and it is easy to spread diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Seabuckthorn tissue culture rapid propagation method of the present invention is:

[0050] (1) Selection and treatment of plant materials: Take the stem section with dormant buds as the test material, first rinse it with tap water, then soak it in detergent solution for 15 minutes, stir continuously during this period, and gently scrub the surface with a soft brush, then use Rinse with tap water, then rinse under running water for 3 hours. Then put it into a sterilized beaker and immediately place it on an ultra-clean workbench, first soak it in 75% alcohol for 30 seconds, then rinse it with sterile water for 3 times, and then soak it in 0.1% mercuric chloride for 4 minutes. Shake continuously during this period, and finally rinse with sterile water 6 times.

[0051] (2) Induction of stem segments with dormant buds: put the stem segments with dormant buds on sterilized filter paper, blot the water dry, cut into 0.8 cm stem segments with single buds and inoculate them o...

Embodiment 2

[0059] Seabuckthorn tissue culture rapid propagation method of the present invention is:

[0060] (1) Selection and treatment of plant materials: Take the stem section with dormant buds as the test material, first rinse it with tap water, then soak it in detergent solution for 10 minutes, stir continuously during this period, and gently scrub the surface with a soft brush, then use Rinse with tap water, then rinse under running water for 2 hours. Then put it into a sterilized beaker and immediately place it on an ultra-clean workbench, first soak it in 75% alcohol for 30 seconds, then rinse it twice with sterile water, and then soak it in 0.1% mercuric chloride for 3 minutes. Shake continuously during this period, and finally rinse with sterile water 5 times.

[0061] (2) Induction of stem segments with dormant buds: put the stem segments with dormant buds on sterilized filter paper to blot the water, cut into 0.5 cm stem segments with single buds and inoculate them on the in...

Embodiment 3

[0069] Seabuckthorn tissue culture rapid propagation method of the present invention is:

[0070] (1) Selection and treatment of plant materials: Take the stem section with dormant buds as the test material, first rinse it with tap water, then soak it in detergent solution for 15 minutes, stir continuously during this period, and gently scrub the surface with a soft brush, then use Rinse with tap water, then rinse under running water for 2.5 hours. Then put it into a sterilized beaker and immediately put it on the ultra-clean workbench, first soak and disinfect with 75% alcohol for 30 seconds, then rinse with sterile water for 3 times, and then immerse and disinfect with 0.1% mercuric chloride for 5 minutes. Shake continuously during this period, and finally rinse with sterile water 6 times.

[0071] (2) Induction of stem segments with dormant buds: put the stem segments with dormant buds on sterilized filter paper to blot the water, cut into 1.1 cm stem segments with single ...

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PUM

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Abstract

The invention provides a tissue-culture rapid-propagation culture medium for sea-buckthorn. The tissue-culture rapid-propagation culture medium comprises an induced culture medium, a multiplication culture medium and a rooting culture medium, wherein the formula of the induced culture medium contains the following components: 1 / 2 MS, 30g / L of saccharose, 6g / L of agar, 1.0mg / L-4.0mg / L of 2, 4-D and0.1mg / L-0.4mg / L of NAA; the formula of the multiplication culture medium contains the following components: 1 / 2 MS, 30g / L of saccharose, 6g / L of agar, 0.5mg / L-3.0mg / L of 6-BA and 0.1mg / L-0.3mg / L of NAA; and the formula of the rooting culture medium contains the following components: 1 / 2 B5, 30g / L of saccharose, 6g / L of agar and 0.1mg / L-0.4mg / L of NAA. The invention further provides a corresponding tissue-culture rapid-propagation method for the sea-buckthorn. By utilizing the culture medium and the rapid-propagation method, the induction rate of stems with dormant buds is 87%-100%, the multiplication rate is 85%-96%, and the rooting rate is 92%-97%.

Description

technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture technology, and in particular relates to a seabuckthorn tissue culture rapid propagation medium and a tissue culture rapid propagation method. Background technique [0002] Seabuckthorn (Latin scientific name: Hippophae rhamnoides Linn.) is a perennial deciduous fruit tree, shrub or small tree. Its fruit, peel, leaf, bark and its processed products contain more than 280 kinds of physiologically active substances, which have strong special effects of anti-inflammatory, bactericidal, pain-relieving and promoting tissue regeneration. Many of the ingredients have shown miraculous therapeutic effects in killing and inhibiting tumor cells, anti-radiation, anti-coagulation, lowering blood pressure, preventing vascular embolism, anti-aging, anti-fatigue, enhancing body vitality and immunity. [0003] Seabuckthorn has luxuriant branches and leaves, well-developed lateral ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 赵英徐航刘伟郑新国胡茵韩晓燕张志刚
Owner 新疆阿勒泰地区林业工作管理站
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