Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polypeptide for localizing proteins on cell membranes and/or cell walls and application thereof

A cell membrane and protein technology, applied in the fields of applications, peptides, desipeptides, etc., can solve the problems of different yeasts

Inactive Publication Date: 2012-05-30
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is evidence that the GPI proteins of filamentous fungi are also involved in protein transport and localization like yeast, but are significantly different from yeast

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polypeptide for localizing proteins on cell membranes and/or cell walls and application thereof
  • Polypeptide for localizing proteins on cell membranes and/or cell walls and application thereof
  • Polypeptide for localizing proteins on cell membranes and/or cell walls and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] 1. Construction of pchiGFP expression vector

[0020] Genomic DNA of Aspergillus fumigatus YJ407 CGMCC 0386 (ZL99105415.6) was extracted. For the extraction method of genomic DNA, refer to the "Molecular Cloning Experiment Guide".

[0021] Design primers ChiG-N and ChiG-C, the nucleotide sequences of ChiG-N and chiG-C are as follows:

[0022] Primer ChiG-N: 5'-G GAATTC GAGCCATTGCCTACATCCTTCAC-3' (the underlined part is the EcoRI enzyme recognition site);

[0023] Primer ChiG-C: 5'-GG GGTACC GCGCGCGCCTCCAGATCAGTAC-3' (the underlined part is the KpnI enzyme recognition site).

[0024] Taking Aspergillus fumigatus genomic DNA as template PCR amplification fragment A, fragment A comprises the 1.5kb promoter (P chiB1 ) and N-terminal signal peptide.

[0025] PCR reaction system: 10×pyrobest Buffer 5 μL, primers ChiG-N and ChiG-C each 1 μL (20 μmol / L), DNA template 0.5 μL (1 μg / μL), dNTPs (each 2.5 mM) 4 μL, pyrobestenzyme (TAKARA, 5 U / μL) 0.5 μL, and finally add wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses polypeptide for localizing proteins on cell membranes and / or cell walls and application thereof. The amino acid sequence of the polypeptide is the sequence 1 in the sequence table. The nucleotide sequence of the DNA molecule for coding the polypeptide is the sequence 2 in the sequence table. The polypeptide is fused with GFP to successfully localize GFP on the cell membranes and / or cell walls. The polypeptide not only provides a method of directional expression for the foreign proteins but also provides tools for localizing the expressed proteins on the cell surfaces to be used for biotransformation. The polypeptide provides a good platform for studying the functions of the gene proteins of aspergillus fumigatus and simultaneously provides a good medium for knowingprotein transport of aspergillus fumigatus.

Description

technical field [0001] The present invention relates to polypeptides for localizing proteins in cell membranes and / or cell walls and applications thereof. Background technique [0002] The GPI (glycosylphosphatidylinositol) modification of proteins in eukaryotic cells is a conservative post-translational glycosylation modification, which can position proteins on the cell membrane and is also a way for protein transport. GPI-modified proteins usually have a hydrophobic N-terminal signal peptide and a C-terminal hydrophobic signal peptide; the N-terminal signal peptide is necessary for protein secretion, while the C-terminal signal peptide is the signal of GPI modification, containing One omega-amino acid at position 17-25 of the C-terminus. Covalent attachment of GPI to ω-sites in the endoplasmic reticulum is catalyzed by transaminase complexes. The GPI proteins of higher eukaryotes are finally located on the cell membrane, while many GPI proteins are also located on the ce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/38C12N15/31C12N15/63C12N1/15C12N1/19C12N1/21C12N15/11
Inventor 金城欧阳浩淼陈晓敏
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com