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Application of compound catechin in resisting drug-resistant strains

A technology for catechins and compounds is applied in the application field of natural compound catechins in anti-resistant strains, which can solve the problems of little antibacterial activity and the like, and achieve the effects of abundant raw material sources, low cost and wide distribution.

Inactive Publication Date: 2011-09-21
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the domestic and foreign research on the antibacterial aspects of catechins is also for the antibacterial screening of total polyphenols or total catechins in plants, especially in tea leaves, and there are few in-depth studies on the antibacterial activity of monomers in vivo and in vitro. ; As for the research on the activity of catechins against drug-resistant strains, there is no report so far

Method used

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  • Application of compound catechin in resisting drug-resistant strains
  • Application of compound catechin in resisting drug-resistant strains
  • Application of compound catechin in resisting drug-resistant strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Study on Anti-Staphylococcus Aureus Activity

[0024] Reagent: catechin, penicillin G (Harbin Pharmaceutical Sixth Factory), Penicillin Serva31749 (Germany serva com.Ltd)

[0025] Strains: Staphylococcus aureus (26112-5) penicillin sensitive strain; Staphylococcus aureus (CMCC 26003 resistant to penicillin G, oxacillin, cefazolin, norfloxacin)

[0026] Medium: Nutrient agar medium (produced by Kangle medium factory of Shanghai Insect Technology Development Company, Chinese Academy of Sciences)

[0027] Screening of antibacterial activity of catechins isolated from the plant Milocarpus by disc diffusion method. After preliminary screening, it is found that the compound catechin of the present invention has no activity against the sensitive strain of Staphylococcus aureus, but has activity against the drug-resistant strain. On this basis, the present invention has carried out re-screening again, and the results are shown in Table 2.

[0028] The inhibitory effect of ta...

Embodiment 2

[0034] This example is the application of catechin in synergistically inhibiting drug-resistant Staphylococcus aureus

[0035] Synergy test

[0036] The combined use of antibiotics and the widespread clinical use of synergists have made the synergistic theory more and more perfect. According to the antibacterial activity of the combined antibiotics, there is a significant difference depending on the components of each antibiotic in the combined drug, and the antibiotic effect after the combined use is judged: when the antibacterial activity after the combined drug is higher than the sum of the antibacterial activities of its components , a synergistic effect is produced.

[0037] Mix catechin and penicillin (Serva31749) in a weight ratio of 1:1; 2:1; 3:1 to find the optimal ratio. The results are shown in Table 3.

[0038] Table 3 The screening of compound and penicillin synergistic optimal ratio

[0039]

[0040] Note: the average value of three tests; D: the diameter ...

Embodiment 3

[0044] This example is the application of catechin in combination with penicillin in anti-drug-resistant Staphylococcus aureus.

[0045] Inhibition rate of strains under synergistic effect

[0046] Mix catechin and penicillin at an optimal ratio of 2:1, make a solution of 4 μg / ml with methanol, add it into a 10ml medium test tube inoculated with strain CMCC 26003 according to the concentration gradient, place it on a shaker, and perform 150 times at 28°C Under the condition of 0, 2, 4, 6, ... 24 hours respectively cultured under the condition of 1 / min, they were taken out and placed in the refrigerator for uniform measurement. At the same time, make a blank control without reagents and a blank control without reagents and inoculated strains, and cultivate them for 12 hours and 24 hours respectively, put 600 μl of the culture test tube to be determined into the EP tube, and process it in a centrifuge at 10000r / min. 200 μl of the supernatant was taken as a blank control, and 20...

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Abstract

The invention discloses application of compound catechin in resisting drug-resistant strains and application of the compound catechin in the synergistic inhibition of drug-resistant staphylococcus aureus, which also relates to application of the compound catechin in the aspect of resisting the drug-resistant staphylococcus aureus when being associated with penicillin for use simultaneously. The compound catechin related to the invention has inhibition activities to the drug-resistant strains of the staphylococcus aureus, and a synergic action experiment of the compound catechin mixed with thepenicillin proves that the compound catechin has the synergistic inhibition activities to the strains of the drug-resistant staphylococcus aureus and the stability of the compound catechin exceeds that of beta-lactamase inhibitor clavulanate potassium. An in-vivo experiment also shows that the catechin and the penicillin have inhibition activities to the strains of the drug-resistant staphylococcus aureus under a synergic action.

Description

technical field [0001] The invention relates to the application of a natural compound catechin in anti-drug-resistant strains, in particular to its application in synergistic anti-drug-resistant bacteria in vivo and in vitro. Background technique [0002] Staphylococcus aureus is one of the main pathogens of nosocomial infection. Staphylococcus aureus can cause skin infection from small to severe pneumonia, meningitis, bone and joint infection, endocarditis and bacteremia that can be fatal. Patients with end-stage renal disease, who are often treated with hemodialysis, are particularly susceptible to S. aureus infection, a leading cause of infection and death in hospitalized patients. With the continuous emergence of new antibiotics, the drug resistance of Staphylococcus aureus has gradually increased, which has become a thorny problem in clinical treatment. At present, the research and development of new antibacterial drugs mainly focus on synthesis, semi-synthesis, and fin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/353A61K31/43A61P31/04
Inventor 康文艺宋艳丽王金梅姬志强
Owner HENAN UNIVERSITY