Bactericidal composition containing iprodione
A composition, the technology of isobactil, applied in the directions of fungicides, biocides, biocides, etc., can solve the problems of easy generation of drug resistance, single action site, etc., to reduce drug dosage, reduce pollution, and delay drug resistance. Effect
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example 1
[0017] Bioassay Example 1: Indoor Toxicity Test of Iprodione and Kresoxim-methyl on Wheat Powdery Mildew
[0018] Test object: Wheat powdery mildew bacteria collected from the field
[0019] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1154.7-2006". Wheat powdery mildew diseased leaves collected from the field were evenly shaken off the conidia above the wheat seedlings for inoculation, and 24 hours later, they were treated with chemicals. See Table 1 for the test chemicals. Select wheat seedlings at the three-to-four-leaf stage with consistent growth potential, and select 3 pots of wheat seedlings for each treatment, and spray them with a Potter spray tower at a pressure of 50PSI, about 5mL per pot. Five concentration gradients were set for each drug. The same amount of water was sprayed as the blank control. After spraying, the wheat seedlings were put into the greenhouse for cultivation. After 7 days, according to the incidence cl...
example 2
[0024] Biological Assay Example 2: Indoor Toxicity Determination of Iprodione and Azoxystrobin Compound to Cucumber Botrytis cinerea
[0025] Test object: Cucumber Botrytis cinerea collected from the field
[0026] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1154.7-2006", mycelium growth rate method. Cucumber Botrytis cinerea was cultivated with PDA medium. When the bacterial colony was just overgrown with the petri dish, a puncher with an inner diameter of 7 mm was punched from the edge, and the resulting mycelium block was used as an inoculum. Take 5 mL of the prepared drug solution and mix them with quantitative 75 mL of sterilized medium to make 4 drug-containing plates, and use the same amount of sterile water mixed with the medium as a control. Put the hyphae upside down and connect them to the center of the plate, and culture them in a 28°C incubator. After 7 days, the colony diameter was measured by the cross method, and the n...
example 3
[0029] The mycelial growth rate was converted into a probability value (y), and the concentration of the drug solution (μg / ml) was converted into a logarithmic value (x), and the toxicity equation and the inhibitory medium concentration EC were calculated by the least square method 50 According to Sun Yunpei's method, the toxicity index and co-toxicity coefficient (CTC) of the drug were calculated. Statistical analysis method is the same as biological assay example 1. The results of the toxicity test are shown in Table 2. Bioassay Example 3: Indoor Toxicity Determination of Iprodione and Trifloxystrobin Compound on Apple Spotted Deciduous
[0030] Test object: Apple spot defoliation fungus collected from the field
[0031] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1154.7-2006", mycelium growth rate method. The test method is the same as the biological assay example 2. The results of the toxicity test are shown in Table 3.
[0032...
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