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Method for preparing bigeminy inactivated vaccine of newcastledisease and H9 hypotype bird flu

A dual-inactivated vaccine and Newcastle disease technology, which is applied in the field of poultry biopharmaceuticals, can solve the problems of high mortality and high morbidity, and achieve the effects of low viscosity, good immune effect, and difficult stratification

Inactive Publication Date: 2009-12-23
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the deficiencies in the prior art, the purpose of the present invention is to provide a preparation method of Newcastle disease and H9 subtype avian influenza dual inactivated vaccine to prevent the high incidence and high mortality caused by current Newcastle disease and H9 subtype of avian influenza. The problem is to minimize the immune interference between the two antigens, reduce the mortality of livestock and poultry, reduce the loss of professional breeding households, and benefit mankind

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] The preparation method of chicken Newcastle disease, H9 subtype bird flu dual combination inactivated vaccine, its preparation steps are:

[0020] 1. Preparation of virus seeds for production: make NDV La Sota strain with sterilized normal saline for 10 -4 Dilute, make AIV HL strain seed with sterilized normal saline for 10 -3 Dilute, inoculate 9-10 day-old SPF chicken embryos respectively, incubate, and harvest; 2. Preparation of virus liquid for making seedlings: use sterilized normal saline for the production of virus seeds of NDV La Sota strain for 10 days -4 Dilute, make AIV HL strain production virus seeds with sterilized physiological saline for 10 -3 Dilute, inoculate 9-10-day-old susceptible chicken embryos, incubate, harvest, respectively adopt the fiber membrane ultrafiltration concentration method for the harvested virus liquid, and make 2 times concentration, use 0.05% formaldehyde solution for NDV La sota and AIVHL strains, Carry out inactivation at 35°C...

Embodiment 2

[0022] The preparation method of chicken Newcastle disease, H9 subtype bird flu dual combination inactivated vaccine, its preparation steps are:

[0023] 1. Preparation of virus seeds for production: make NDV La Sota strain with sterilized normal saline for 10 -4 Dilute, make AIV HL strain seed with sterilized normal saline for 10 -3 Dilute, inoculate 9-10 day-old SPF chicken embryos respectively, incubate, and harvest; 2. Preparation of virus liquid for making seedlings: use sterilized normal saline for the production of virus seeds of NDV La Sota strain for 10 days -4 Dilute, make AIV HL strain production virus seeds with sterilized physiological saline for 10 -3 Dilute, inoculate 9-10-day-old susceptible chicken embryos, incubate, harvest, respectively adopt the fiber membrane ultrafiltration concentration method for the harvested virus liquid, and make 2 times concentration, use 0.1% formaldehyde solution for NDV La sota and AIVHL strains, Carry out inactivation at 37°C ...

Embodiment 3

[0025] The preparation method of chicken Newcastle disease, H9 subtype bird flu dual combination inactivated vaccine, its preparation steps are:

[0026] 1. Preparation of virus seeds for production: make NDV La Sota strain with sterilized normal saline for 10 -4 Dilute, make AIV HL strain seed with sterilized normal saline for 10 -3 Dilute, inoculate 9-10 day-old SPF chicken embryos respectively, incubate, and harvest; 2. Preparation of virus liquid for making seedlings: use sterilized normal saline for the production of virus seeds of NDV La Sota strain for 10 days -4 Dilute, make AIV HL strain production virus seeds with sterilized physiological saline for 10 -3 Dilute, inoculate susceptible chicken embryos of 9 to 10 days old respectively, incubate, harvest, respectively adopt the fiber membrane ultrafiltration concentration method for the harvested virus liquid, and make 2 times concentration, use 0.15% formaldehyde solution for NDV La sota and AIVHL strains, Carry out ...

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Abstract

The invention discloses a method for preparing a bigeminy inactivated vaccine of newcastledisease and H9 hypotype bird flu. The method is characterized by comprising the following steps: (1) preparing virus seed for production; (2) preparing virus liquid for preparing vaccine; (3) evenly mixing virus liquid of the inactivated NDV LaSota strain and AIV HL strain with virus liquid of AIV HL strain, wherein the virus liquid of the inactivated NDV LaSota strain is condensed by 2 times; adding 97 to 99 volume portions of mixed virus liquid into 1 to 3 volume portions of tween-80, and mixing the substances to prepare aqueous phase; mixing 96 volume portions of white oil, 4 volume portions of span-80 and 2 volume portions of aluminum stearate to prepare oil phase; and (4) mixing the aqueous phase and the oil phase in a mixed ratio of 1:1.5-2.5, and emulsifying the mixture to prepare the bigeminy inactivated vaccine of the newcastledisease and the H9 hypotype bird flu. The prepared bigeminy inactivated vaccine of the newcastledisease and the H9 hypotype bird flu has the advantages of capability of effectively preventing the current morbidity caused by the newcastledisease and the H9 hypotype bird flu, good effect and the like.

Description

Technical field: [0001] The invention relates to the technical field of poultry biopharmaceuticals, in particular to a preparation method of a dual inactivated vaccine for Newcastle disease and H9 subtype avian influenza. Background technique: [0002] Newcastle disease, commonly known as chicken plague, is a highly contagious infectious disease caused by chicken Newcastle disease virus. It mainly infects the respiratory tract and digestive tract. Chickens of all ages can be infected, and young chickens are the most serious, with a morbidity and mortality rate of up to 80%. Above; Avian influenza is an infection and / or disease syndrome of poultry caused by type A influenza virus. Infected house-fed poultry, including poultry, can exhibit clinical symptoms, respiratory disease, decreased egg production, acute hemorrhagic disease with high mortality, and subclinical symptoms, mild respiratory infections to asymptomatic Symptoms are poisonous and other popular forms. Accordin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/14A61P31/16
Inventor 乔荣岑习向锋孙进忠荣骏弓闵亚杰
Owner PU LIKE BIO ENG
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