Electrochemical method for measuring toxicity effect of multi-walled carbon nanotubes
A multi-walled carbon nanotube, electrochemical technology, applied in the direction of biochemical equipment and methods, electrochemical variables of materials, measurement/inspection of microorganisms, etc., can solve the problems of large equipment volume, complicated operation, unfavorable monitoring, etc., and achieve equipment Lightweight, easy-to-operate effect
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Embodiment 1
[0061] 0.1mol·L -1 25 mL of Tris-HCl buffer solution (pH=7.5) was placed in the three-electrode system, and the supporting electrolyte was 0.15 mol L -1 KCl, constant temperature (25°C), nitrogen deoxygenation for 10min, add 35mg L -1 MWCNTs (d=10-20nm, length 1-2μm), stirred rapidly for 2min and then sonicated for 10min, maintained nitrogen atmosphere, injected 30μL of LDH liquid, stirred for a certain period of time, added pyruvic acid (8.0×10 -4 mol L -1 ) and NADH (2.0×10 -4 mol L -1 ), record NAD under different reaction times + Differential pulse voltammetry reduction peak current i p，NAD + , change the stirring time after injecting the LDH liquid and carry out a series of experiments, and compare with the LDH reaction system whose stirring time is 5min in Comparative Example 1, the results are as follows Figure 6 shown. It can be seen from the figure that after LDH interacts with MWCNT for 5 minutes, i p，NAD + The decline of relative to no carbon nanotubes i...
Embodiment 2
[0065] 0.1mol·L -1 25 mL of Tris-HCl buffer solution was placed in the three-electrode system, and the supporting electrolyte was 0.15 mol L -1 KCl, constant temperature (25°C), nitrogen deoxygenation for 10min, add 35mg L -1 MWCNTs (d=10-20nm, length 1-2μm), stirred rapidly for 2min and then sonicated for 10min, maintained nitrogen atmosphere, injected 30μL of LDH liquid, stirred for 5 minutes, added pyruvic acid (8.0×10 -4 mol L -1 ) and NADH (2.0×10 -4 mol L -1 ), record NAD under different reaction times + Differential pulse voltammetry reduction peak current i p，NAD + , change the pH value of the buffer solution, and conduct a series of experiments, the results are as follows Figure 8 shown.
[0066] Depend on Figure 7 , 8 It can be seen that the two enzymatic systems, the buffer solution with similar pH, and the same reaction time p，NAD + This may be due to the fact that acidity has little or no effect on the solubility of MWCNTs. When the pH value is lowe...
Embodiment 3
[0068] 0.1mol·L -1 25 mL of Tris-HCl buffer solution (pH=7.5) was placed in the three-electrode system, and the supporting electrolyte was 0.15 mol L -1 KCl, constant temperature (25°C), nitrogen deoxygenation for 10min, add MWCNTs (d=10-20nm, length 1-2μm), stir rapidly for 2min, then sonicate for 10min, maintain nitrogen atmosphere, inject 30μL of LDH liquid, stir for 5 minutes , add pyruvic acid (8.0×10 -4 mol L -1 ) and NADH (2.0×10 -4 mol L -1 ), record NAD under different reaction times + Differential pulse voltammetry reduction peak current i p，NAD + , changing the amount of MWCNTs added, and conducting a series of experiments, the results are as follows Figure 9 , 10 shown. The results show that: after adding a small amount of MWCNT aqueous solution i p，NAD + The peak current changes more obviously, i p，NAD + After 12 minutes, the current began to have a significant difference, which may be due to the small solubility of MWCNT in water, but only uniformly...
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