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Method for producing blue-ear disease vaccine by culturing sensitive cell

A technology for sensitive cells and PRRS, applied in biochemical equipment and methods, tissue culture, microorganisms, etc., can solve problems such as low degree of automation, large product batch differences, and unstable quality of cells and PRRS vaccines.

Inactive Publication Date: 2009-12-30
扬州优邦生物药品有限公司
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AI Technical Summary

Problems solved by technology

This method has a low degree of automation and high labor intensity, and because of the uncontrollable environment of the spinner bottle cell culture, the quality of the cells and the PRRS vaccine is unstable, and the product batches vary greatly

Method used

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  • Method for producing blue-ear disease vaccine by culturing sensitive cell

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, microcarrier culture Marc145 cell production PRRS vaccine in 50 liters of bioreactors

[0027] Microcarrier: cytodex-1 (GE Life Sciences, Cat No.17-0485-03)

[0028] Porcine reproductive and respiratory syndrome virus: NVDC-JXA1 strain.

[0029] Medium: Marc145 cell reactor high-density medium (Beijing Qingda Tianyi Biotechnology Co., Ltd., Cat No.MD900, Lot No.080310)

[0030] In a 50-liter bioreactor, cytodex-1 was rinsed with PBS, soaked several times, and after autoclaving, Marc145 cells were inoculated into the bioreactor, and the cells grew to 2×10 6 After the cell density of cells / ml, perfusion culture was carried out until the viable cell density reached 6×10 6 After the cells / ml, the virus solution was replaced, and the porcine reproductive and respiratory syndrome virus was inoculated according to the virus multiplicity of infection MOI=0.015. Then, maintain the nutritional conditions required for virus propagation and the pH value of 6.9-7.3 ...

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Abstract

The invention provides a method for producing a blue-ear disease vaccine by culturing a sensitive cell. The method comprises the following steps: 1) culturing the sensitive cell on a microcarrier in a bioreactor; 2) inoculating a porcine reproductive and respiratory syndrome virus to the sensitive cell cultured on the microcarrier; 3) continuously culturing the sensitive cell; and 4) obtaining virus solution to produce the blue-ear disease vaccine. The method produces the blue-ear disease vaccine by culturing the sensitive cell in the bioreactor by applying microcarrier culture technology. The microcarrier provides larger surface area, the cell can reproduce to generate higher cell density, and automatically controlled culture environment parameters of the bioreactor ensure that the cell growth and virus propagation can be in an excellent environment, so the method can improve the virus titer, and ensures more stable vaccine quality.

Description

technical field [0001] The invention relates to a method for cultivating cells to produce vaccines, in particular to a method for cultivating sensitive cells to produce PRRS vaccines. Background technique [0002] PRRS is a contagious disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) infection. Pigs of various ages can be infected, and the main clinical manifestations are reproductive disorders in sows and respiratory symptoms in piglets. Reproductive disorders of sows can be manifested as miscarriage, stillbirth and weak piglets, and the death rate of piglets after birth increases; respiratory symptoms of suckling piglets mainly manifest as symptoms of pneumonia such as high fever and dyspnea. There is no specific drug therapy for the disease at present, and the method of compulsory immunization with vaccines is mainly adopted. [0003] At present, the production of PRRS vaccine is mainly carried out by the process of cultivating Marc145 cells ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12C12N5/06A61P31/14
Inventor 陈文庆刘俊生王建超
Owner 扬州优邦生物药品有限公司
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