A method for measuring raffinose and stachyose content in food
A technology of stachyose and raffinose, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unrealistic production and testing, low quality of operators, backward instruments, etc., and achieve low requirements for instruments and equipment, repeatability Good, cheap results
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Embodiment 1
[0032] Drawing of standard curves for raffinose and stachyose.
[0033] (1) Prepare raffinose or stachyose standard solution with a certain gradient concentration;
[0034] (2) The raffinose or stachyose standard solution is applied to the thin-layer plate by strip spotting, the length of the strip is 2cm, and the spotting volume is 5 μl, and it is developed at a constant temperature of 30° C. in the chromatography cylinder;
[0035] (3) After unfolding, take it out, dry it, spray the color developer, put it in an oven at 90°C for 10 minutes, take out the color plate after natural cooling, and take 2cm×4cm in the color area of raffinose or stachyose The area of the band was collected in a centrifuge tube, added 4mL of water, shaken evenly, ultrasonicated for 20min, taken out, centrifuged at 4000r / min for 8min, and the supernatant was taken. The developer in the chromatography cylinder is acetonitrile: glacial acetic acid: water (5:4:1.5), the color developer is aniline-di...
Embodiment 2
[0042](1) Take 200.0mL of cola drink and place it in an evaporating dish, remove carbon dioxide on a water bath, transfer it into a 250mL volumetric flask, wash the evaporating dish with water, put the washing liquid into the volumetric flask, add water to the mark, mix well and set aside (2) Draw 5 μ L of the above-mentioned liquid on the thin-layer plate to spot the sample. The strip spotting is used for spotting. The length of the strip is 3 cm. It is expanded in the developing agent, and the spotting plate is placed in the chromatographic cylinder. (3) After unfolding, take it out and blow it dry, spray the color developer on it, put it in an oven at 85°C for 10 minutes, take out the color plate after natural cooling, and mix it with raffinose and stachyose For the color-developing area, take 2cm×4cm spot bands, collect them in a centrifuge tube, add 3mL of water, shake well and ultrasonicate for 20min, take them out, centrifuge at 4800r / min for 10min, and take the supernat...
Embodiment 3
[0047] (1) Weigh 75g of ice cream in a Soxhlet extractor, remove the fat from it by Soxhlet extraction, the extractant is petroleum ether, and the extraction temperature is 80°C; (2) Put the ice cream after fat removal into a 250mL volumetric flask Add 50mL of water, slowly add 4mL of zinc acetate solution and 5mL of potassium ferrocyanide solution, add water to the mark, mix well, precipitate, let stand for 30min, filter with dry filter paper, and use the filtrate for later use. (3) Draw 5 μ L of the above-mentioned filtrate and place samples on the thin-layer plate. The strips are used for spotting the samples. The length of the strips is 3 cm. The strips are developed in the developer, and the spotting plate is placed in the chromatography cylinder. , at a constant temperature of 25°C; (4) After unfolding, take it out and blow it dry, spray the color developer on it, place it in an oven at 85°C for 10 minutes, take out the color plate after natural cooling, and test it under...
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