Bacillus subtilis and application thereof in biological feed additives

A technology of Bacillus subtilis and biological feed, which is applied in the direction of animal feed, animal feed, and methods based on microorganisms, and can solve the problems of insufficient antibacterial effect, poor resistance to digestive tract physiological environment and feed processing, and poor safety Reliability and other issues, to achieve the effect of promoting intestinal micro-ecological balance, increasing weight gain rate and feed conversion rate, and no drug withdrawal period

Inactive Publication Date: 2010-02-03
惠飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some feed companies are actively looking for and researching new green and environmentally friendly microbial feed additives, but such additives on the market

Method used

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  • Bacillus subtilis and application thereof in biological feed additives
  • Bacillus subtilis and application thereof in biological feed additives
  • Bacillus subtilis and application thereof in biological feed additives

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1 Primary screening and separation and purification of strains

[0030] To slaughter healthy suckling pigs, take the contents of the stomach, duodenum, jejunum, ileum, cecum and colon close to the mucosa in a certain amount of sterile water, stir and dilute 10 times, and inoculate it in BPY liquid medium. Incubate in a constant temperature shaker at 35°C at 200 rpm for 30 hours.

[0031] Boil the above-mentioned bacterial liquid in boiling water for 10 minutes, and then streak culture on a BPY medium plate, pick out various colonies with different shapes and streak culture and separation, and check whether there are spores and whether they are single colonies under a microscope. The 40 isolated single colonies and spores were respectively inoculated with BPY medium and cultured on the slope of the test tube at 35°C for 30h, and stored in the refrigerator at 4°C until use.

[0032] The composition of BPY medium is as follows: beef extract 0.5g, peptone 1g, sodium chlor...

Example Embodiment

[0033] Example 2 Screening of enzyme production capacity

[0034] The casein hydrolysis plate was used to screen the 40 strains of spores screened in Example 1 for high protease-producing excellent strains. Observe the size of the transparent circle of 40 strains of spore protease, and select 5 strains of spores with large transparent circle (that is, high protease-producing activity) for the next step of screening.

[0035] Casein plate medium: beef extract 8g, yeast powder 2g, peptone 5g, sodium chloride 2g, casein 4g, agar 20g, distilled water 1000ml, pH 7.2-7.4.

Example Embodiment

[0036] Example 3 Screening of strains resistant to the physiological environment of the digestive tract

[0037] The 5 strains screened out in Example 2 were screened into excellent strains resistant to the physiological environment of the digestive tract.

[0038] (1) The preserved slant strains were inoculated in a test tube containing 10ml of BPY liquid medium, incubated at 35°C for 30h, and inoculated into 9.0ml of artificial simulated gastric juice with pH values ​​of 2.0, 3.0, 4.0 at 10%, 0h Counting was used as a control, samples were taken at 2h and 6h with PBS buffer (PH7.0, 10mmol / L) in a series of 10-fold dilutions, plate viable bacteria technology was performed, survival rate was calculated, and excellent bacteria with survival rate above 3% were screened.

[0039] Preparation of artificial simulated gastric juice: Measure 16.4ml of concentrated hydrochloric acid, add distilled water to 1000ml, make basic artificial gastric juice, adjust pH to 2.0, 3.0, 4.0 with hydrochlo...

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Abstract

The invention belongs to the field of feed additive and discloses a bacillus subtilis and the application thereof in biological feed additives. The bacillus subtilis HF-A2 related in the invention ispreserved in Common Microorganism Center, China Committee of Culture Collection for Microorganisms on August 19th, 2009, with a preservation number of CGMCC No. 3241. The bacillus subtilis HF-A2 (CGMCC No. 3241) is obtained by selecting content matters collected from gastrointestinal parts of a healthy anthony pig, which are close to mucosa. The strain of the bacillus subtilis has a strong capability in resisting the physical environment of the alimentary canal and resisting processing, performs well in inhibiting bacteria and deodorization and is safe and reliable. The biological feed additives prepared from the strain can inhibit harmful microorganisms in the alimentary canal, replace antibiotics and chemical drugs, remarkably enhance the immunity of livestock and poultry, promote the healthy growth of the animals, reduce the odor of wastes and purify the raising environment.

Description

technical field [0001] The invention belongs to the field of feed additives, and relates to a bacillus subtilis and its application in biological feed additives. Background technique [0002] In recent years, my country's animal husbandry production has developed rapidly, and large-scale and intensive livestock and poultry farms have shown a sharp upward trend. Residues, high breeding costs, etc., have seriously restricted the further development of the breeding industry. In order to solve these problems in breeding, it is urgent to develop a new type of feed additive, which can not only greatly reduce the odor of animal manure, but also improve the feed conversion rate, improve the immunity and disease resistance of animals, and promote their health and rapid development. grow. According to the theory of microecology, after entering the intestinal tract, the live bacterial preparations of beneficial bacteria such as lactic acid bacteria can promote the growth of beneficial...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/125A23K10/18
Inventor 刘小勇王有智惠飞王大伟魏晶惠大帅肖淑红翁万山
Owner 惠飞
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