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Microinjection method for regulating and controlling grain weight of soybean seeds by exogenous abscisic acid

A technology of abscisic acid and injection, which is applied in the field of microinjection, can solve the problems of unstable absorption of exogenous hormones, loss of exogenous hormones, etc., and achieve good absorption effect, obvious effect, and obvious treatment effect

Inactive Publication Date: 2011-07-20
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing research method of spraying exogenous hormones to regulate soybean yield is susceptible to the loss of exogenous hormones caused by environmental conditions, and the absorption of exogenous hormones is unstable due to the influence of soybean growth period and metabolic level in the body. problem, and provide a microinjection method for exogenous abscisic acid to regulate soybean grain weight

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0007] Specific embodiment 1: In this embodiment, the microinjection method of exogenous abscisic acid regulation and control of soybean grain weight is carried out as follows: 1. Configuration of buffer solution: Weigh 38 to 42 grams of potassium dihydrogen phosphate and 1.2 to 1.8 grams of Dissolve boric acid in 1 liter of pure water; 2. Preparation of abscisic acid injection: Accurately weigh 0.6-0.7 mg of abscisic acid standard sample with a ten-thousandth balance, put it in a plastic EP tube with a scale of 1 ml, and take the buffer solution Dissolve the solid and set the volume to 1ml, shake well with a vortex; 3. Injection: Use a micro-syringe to extract 15 μl of abscisic acid injection from the EP tube containing abscisic acid injection, and select the middle and upper parts of soybean plants at the soybean R5 stage For a pod with three seeds at the node, inject it into the middle of the two cotyledons of the second seed along the dorsal seam of the pod at a distance of...

specific Embodiment approach 2

[0009] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, 40 grams of potassium dihydrogen phosphate and 1.2 to 1.8 grams of boric acid are weighed and dissolved in 1 liter of pure water. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0010] Specific embodiment three: the difference between this embodiment and specific embodiment one is: In step one, 40 grams of potassium dihydrogen phosphate and 1.5 grams of boric acid are dissolved in 1 liter of pure water. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a microinjection method for regulating and controlling grain weight of soybean seeds by exogenous abscisic acid and relates to the microinjection method for regulating and controlling the grain weight of the soybean seeds by exogenous hormones.The microinjection method can solve the problems that the existing research method for regulating and controlling the yield of soybeans by spraying exogenous hormones is easy to be affected by environment conditions for causing loss of the exogenous hormones, and easy to be affected by growing period and in vivo metabolism level of the soybeans for causing instable absorption of the exogenous hormones. The microinjection method for regulating and controlling the grain weight of the soybean seeds by the exogenous abscisic acidis carried out according to the following steps: 1, preparing buffer solution; 2, preparing abscisic acid injection; 3, injection; and 4, weighing the grain weight. The adoption of the microinjectionmethod for regulating and controlling the grain weight of the soybean seeds by the exogenous abscisic acid can avoid impacts of rainfall, temperature, humidity, wind direction and other external environments, significantly reduce the concentration of the exogenous abscisic acid and avoid the loss of the exogenous abscisic acid. The method has important theoretical and practical significance for researching the physiological and ecological process and the mechanism of regulating and controlling the grain weight of the soybeans by the exogenous hormones.

Description

technical field [0001] The invention relates to a microinjection method for regulating soybean grain weight by exogenous hormones. Background technique [0002] The domestic and foreign research methods of applying exogenous hormones to regulate soybean yield mainly take the measure of spraying, which is carried out by spraying exogenous hormones to leaves or flower pods. Although this method is simple and feasible, it is strongly affected by environmental conditions. For example, the influence of rainfall, temperature, humidity and wind direction often leads to the loss of sprayed exogenous hormones, making the effect of exogenous hormones on soybeans insignificant. At the same time, this method is also affected by the soybean growth period and the metabolism level in the body, which leads to the unstable absorption of exogenous hormones by soybean leaves or flower pods. Contents of the invention [0003] The purpose of the present invention is to solve the problem that...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06A01G1/00
Inventor 刘兵王程刘晓冰金剑
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S