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Protective freezedrying powder of marine microorganism neuzyme and preparation method

A technology of marine microorganisms and manufacturing methods, applied in the field of manufacturing of marine microorganism lysozyme protected freeze-dried powder, to achieve the effects of reduced water content, additional cost savings, and high activity

Inactive Publication Date: 2011-08-17
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the key problem is how to obtain a stable and highly active enzyme preparation

Method used

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  • Protective freezedrying powder of marine microorganism neuzyme and preparation method
  • Protective freezedrying powder of marine microorganism neuzyme and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Mix trehalose with a concentration of 0.5 mol / L and Tween 80 with a mass concentration of 20 g / L in a pre-cooled container in equal volume, and then add it at a volume ratio (volume ratio of the above mixed solution to lysozyme solution) of 1:1 Marine microbial lysozyme solution, mixed evenly and pre-frozen for 3 hours; concentration of marine microbial lysozyme solution 100g / L;

[0030]Transfer the above-mentioned pre-frozen materials to the sieve tray of the freeze dryer, and perform primary freeze-sublimation drying in the freezer chamber. The primary freeze-sublimation drying temperature is -45°C, the vacuum pressure is 13Pa, and the freezing time is 30 hours, so that the moisture in the material is directly After sublimation to almost complete sublimation, transfer to secondary freeze-sublimation drying. In secondary freeze-sublimation drying, the temperature is raised from -45°C to 20°C at a heating rate of 0.2°C / min within 5-8 hours, and the pressure is maintained...

Embodiment 2-3

[0032] The freeze-drying procedure was the same as that in Example 1, except that maltose and sucrose were used to replace trehalose to obtain marine microbial lysozyme-protected freeze-dried powders of maltose-Tween 80 and sucrose-Tween 80 composite protective agents.

Embodiment 4

[0033] Embodiment 4, the steps are the same as in Embodiment 1, except that the molar concentration of trehalose is 0.7 mol / L, and the marine microbial lysozyme solution is 50 g / L.

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Abstract

The invention relates to a preparation method of protective freezedrying powder of marine microorganism neuzyme, comprising the following steps: evenly mixing compound protective agent of disaccharide and tween 80 with marine microorganism neuzyme according to a specified concentration and a given volume ratio in a precooling container, prefreezing for 3-5 hours at the temperature of minus 70-minus 90 DEG C, and then carrying out primary and secondary freeze drying in a freezing chamber of a freeze drying machine, and finally obtaining the protective freezedrying powder. The method obtains the protective freezedrying powder with stability, homogenization, high activity, convenient transportation and storage (room temperature) and low cost, and the protective freezedrying powder can be used for preparing various marine microorganism neuzyme preparations.

Description

technical field [0001] The invention relates to the field of industrial preservation of marine microbial enzymes, in particular to a method for manufacturing uniform, stable and highly active marine microbial lysozyme protected freeze-dried powder. Background technique [0002] The vast ocean is the cradle of life on the earth. It covers 71% of the earth's surface area, and the total amount of seawater accounts for 97% of the total water on the earth. The effective area of ​​the unused ocean and coast is 5-10 times larger than that of land. Marine microorganisms are important members of marine life. [0003] The ecological environment of the ocean is very unique, including high salinity, high pressure, low temperature (especially in the deep sea), low light, oligonutrients, etc., as well as extreme environments with no light and local high temperature. Microorganisms from the ocean are extremophiles adapted to extreme environments, and can often produce new active substance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/34A61K47/26A61K38/46A61K9/19A61P31/04
Inventor 孙谧王跃军刘均忠郑媛纪存鹏郝建华
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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