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Method for using female sterility gene fst in hybrid rice breeding

A technology for hybrid rice and male sterile lines, which is applied in the field of hybrid rice breeding with the female sterile gene FST, can solve the problems of mixed maintainer and restorer lines, avoid the cost of removing impurities, eliminate mechanical mixing, and accelerate the promotion and application The effect of the process

Inactive Publication Date: 2012-02-01
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the United States and other countries where the degree of mechanization is high and the artificial breeding system is replaced by machine operation, the mixing of maintainer and restorer lines is more serious.

Method used

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  • Method for using female sterility gene fst in hybrid rice breeding

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Seed production system (MS+FS) of the present invention, method is as follows:

[0015] 1) Propagate the rice male sterile line Heline 42A according to the current three-line method;

[0016] 2) Propagate the female sterile line Ansanbyeo / G39 / / W1944 (female sterile line, AGW-fsfs for short) by means of perennial root asexual reproduction;

[0017] 3) The rice male sterile line and the female sterile line are planted in the same field, the planting specification is 42A: AGW-fsfs=12:2, no impurities are removed during the planting period, artificial pollination is carried out to assist pollination during flowering, and the rest The cultivation management method is the same as that of ordinary seed production; during seed production, the female parent, the pollen recipient, is the male sterile line, the male parent, the pollen donor, is the female sterile line (FS), and the female sterile line pollens the male sterile line, Harvest seeds as hybrids;

[0018] 4) When matu...

Embodiment 2

[0024] Seed production system (MS+FS) of the present invention, method is as follows:

[0025] 1) Propagate the rice male sterile line Heline 42A according to the current three-line method;

[0026] 2) Cultivate the transgenic female sterile line Ansanbyeo / G39 (conditional expression dual-purpose female sterile line, referred to as AG-fsfs, genotype is RfRf-fsfs-CIP::FST), according to the chemical reagents required for promoter expression, During the flowering period, artificially spray the florigen, regulate the expression of FST gene to restore the female fertility, and complete the self-reproduction of the female sterile line;

[0027] 3) Mix the seeds of 42A and AG-fsfs (conditional expression dual-purpose type) according to the ratio of 6:1, mix and sow the mixed seeds according to the conventional seedling raising and planting specifications, do not remove impurities during planting, and do not artificially perform flowering Pollination is assisted by pollination, and ...

Embodiment 3

[0034] Seed production system (MS+FS) of the present invention, method is as follows:

[0035] 1) Propagate the rice male sterile line Diantype 1A according to the current two-line method;

[0036]2) Propagate the female sterile line Nan 29 / G39 / / W1944 (female sterile line, NGW-fsfs for short) by means of perennial root asexual reproduction;

[0037] 3) Plant the male sterile line and the female sterile line of rice in the same field, the planting specification is Dian type 1 No. A:NGW-fsfs=12:2, do not remove impurities during planting, and artificially catch pollination to assist pollination during flowering , and the rest of the cultivation and management methods are the same as ordinary seed production; when producing seeds, the female parent, the pollen recipient, is the male sterile line, the male parent, the pollen donor, is the female sterile line (FS), and the female sterile line pollens the male sterile line line, the harvested seeds are hybrids;

[0038] 4) When ma...

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Abstract

The invention relates to a method for using female sterility gene FST in hybrid rice breeding, and belongs to the field of breeding in the field of agricultural biotechnology. The technical scheme of the present invention is to reproduce the male sterile line according to the three-line or two-line method; the reproduction of the rice female sterile line can be completed by the perennial root asexual reproduction method, or the conditionally expressed dual-purpose transgenic female sterile line can be cultivated, By artificially regulating the expression of the FST gene, the female fertility is restored and self-reproduction is completed; when producing seeds, the male sterile line and the female sterile line are planted in the same field, the pollen recipient is the male sterile line, and the pollen donor is the female CMS, the female CMS pollinates the male CMS, and the seeds are harvested as hybrids. The invention utilizes male sterility (MS) and female sterility (FS) simultaneously to construct a "MS+FS" seed production mode, which is suitable for mechanized operation, prevents mechanical mixing of restorer lines, and can accelerate the popularization and application process of hybrid rice.

Description

technical field [0001] The invention relates to a method for using female sterility gene FST in hybrid rice breeding, which belongs to the field of agricultural biotechnology, and more specifically belongs to the field of crop breeding. Background technique [0002] Rice is the second largest cereal crop in the world, and nearly 50% of the global population depends on it as the main source of nutrition. Although in rice breeding, the discovery and utilization of the dwarf gene and cytoplasmic male sterility gene in the 1950s and 1970s has greatly increased the per unit and total yield of rice in the world, and solved the problem of food and clothing for a considerable number of people. However, in the past 40 years, the rapid growth of the global population is also a problem that cannot be ignored. Since the 1970s, the growth rate of global rice production has not been as fast as the population growth rate. Since 2002, the consumption has exceeded the production and the inve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04C12N15/29C07K14/415
Inventor 陈丽娟李东宣李成云谭学林徐学洙朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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