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Method of producing purified rebaudioside a compositions using solvent/antisolvent crystallization

A technology for rebaudioside and composition, which is applied in the directions of food preparation, sugar derivative preparation, chemical instrument and method, etc., can solve the problem of not separating pure sweet glycosides and the like

Inactive Publication Date: 2014-08-06
CARGILL INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This step separates a mixture of sweet glycosides but not a single pure sweet glycoside such as rebaudioside A

Method used

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  • Method of producing purified rebaudioside a compositions using solvent/antisolvent crystallization
  • Method of producing purified rebaudioside a compositions using solvent/antisolvent crystallization
  • Method of producing purified rebaudioside a compositions using solvent/antisolvent crystallization

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Experimental program
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Effect test

Embodiment 1

[0081] First, the initial composition 70 RA80 (see Table 1) was dissolved at 30% by weight in a solvent composition containing 50% by weight ethanol and 50% by weight water. The resulting solution was allowed to equilibrate overnight at room temperature. After equilibration, the antisolvent methanol was added to the sweetening glycoside composition in the amounts shown in Table 2 to induce crystallization. After addition of the antisolvent, crystallization was allowed to proceed overnight at room temperature. After crystallization, the solution was filtered and the resulting filter cake was washed with 2 weights of ethanol. The filter cake and supernatant samples were then dried, and the dried filter cake was analyzed by HPLC to determine the glycoside composition. The results obtained are reported in Table 2.

[0082]

Embodiment 2

[0084] First, the starting materials shown in Table 3 were dissolved at 30% by weight in a solvent composition containing 50% by weight of ethanol and 50% by weight of water. The resulting solution was allowed to equilibrate overnight at room temperature. After equilibration, 2 volumes of antisolvent were added to the sweetening glycoside composition to induce crystallization. After addition of the antisolvent, crystallization was allowed to proceed overnight at room temperature. The addition of 2 volumes of antisolvent was observed to produce crystallization in the 80% concentration of the rebaudioside A starting material, but not in sweet glycoside compositions prepared from 60% or 40% rebaudioside A starting compositions crystallization. An additional volume of antisolvent was added to the glycoside compositions prepared from the 60% and 40% rebaudioside A starting compositions. The extra volume of ethanol antisolvent caused crystallization to occur in two of the three s...

Embodiment 3

[0087]RA20 (see Table 1) was dissolved in a solution of 50% ethanol and 50% water to yield a solution containing 30% by weight RA20. The solution was diluted with 3 times the weight of methanol, filtered, and washed with 2 times the weight of methanol. The crystals accounted for 56% by weight of the original material and were rich in steviol glycosides. What remains is the filtrate and is rich in rebaudioside A. The purity of the obtained crystals and filtrate is given in Table 4.

[0088] Table 4

[0089]

[0090] * Other = sum of peaks between rebaudioside A and rebaudioside B, which is believed to include rebaudioside D and steviolbioside

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Abstract

The invention provides methods of purifying rebaudioside A from a mixture comprising glycosides of the plant Stevia rebaudiana. The methods of the invention are useful for preparing highly pure rebaudioside A compositions from crude Stevia starting compositions that are typically considerably lower in rebaudioside A concentration. The highly pure rebaudioside A compositions are useful as non-caloric sweeteners in edible or chewable compositions such as food, beverages, medicine, candy, chewing gum, and the like.

Description

[0001] Cross References to Related Applications [0002] This patent application claims U.S. Provisional Patent Application Serial No. 60 / 881798, filed January 22, 2007, entitled Process for Preparing Purified Rebaudioside A Compositions Using Solvent / Antisolvent Crystallization, and the December 2007 Priority to U.S. Provisional Patent Application Serial No. 61 / 008163, entitled Process for Preparing Purified Rebaudioside A Compositions Using Solvent / Antisolvent Crystallization, filed on March 19, which is incorporated herein in its entirety middle. technical field [0003] The present invention relates to a method for purifying rebaudioside A from the crude extract of the plant Stevia rebaudiana by solvent / antisolvent crystallization. Background technique [0004] The species Stevia rebaudiana ("Stevia") has been the subject of significant research and development efforts in the purification of certain naturally occurring sweet glycosides of Stevia (Stevia) that have poten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/236C07H1/08A23L27/30
Inventor J·J·哈恩J·C·埃万斯A·S·迈尔森T·奥曼T·A·鲁尼穆斯C·A·泰勒K·M·斯托罗
Owner CARGILL INC
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