Fungal bed cultivation method of hon-shimeji mushroom

A cultivation method and fungus bed technology, which is applied in the field of fungus bed cultivation of Benzhanji mushrooms, can solve the problems of high price, low yield of fruiting body, high cost of culture medium, etc., and achieve the effect of excellent shape

Inactive Publication Date: 2010-06-23
TAKARA HOLDINGS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, since the price of wheat used in the medium used in the method of Patent Document 1 (JP-A-07-115844) is high, the cost of the medium is high
In addition, the yield of fruiting bodies formed in the inventor's method of Patent Document 2 (JP-A-06-153695) is low, so it has not yet reached the level of industrial production

Method used

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  • Fungal bed cultivation method of hon-shimeji mushroom
  • Fungal bed cultivation method of hon-shimeji mushroom
  • Fungal bed cultivation method of hon-shimeji mushroom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The mycelium of Lyophyllum shimeji La 01-27 (FERM BP-10960) was inoculated in 100 ml of PGY liquid medium (composition: glucose 2.0% (w / v), peptone 0.2% (w / v), yeast extract 0.2% (w / v), KH 2 PO 4 0.05% (w / v), MgSO 4 ·7H 2(00.05% (w / v)), cultured at 25° C. for 7 days under the condition of shaking (100 rpm). 2 ml of the culture mixture was cultured in 200 ml of the same medium, and cultured for 7 days under shaking (100 rpm). Subsequently, the entire culture mixture was inoculated in a fermenter (manufactured by Komatsukawa Seisakusho Co., Ltd.) having a volume of 200 liters and containing 160 liters of the same medium, and cultured with stirring for 6 days (stirring speed: 100 rpm, ventilation rate: 25 liters) / min), so as to prepare a liquid seed culture. On the other hand, crushed corn (manufactured by Iisaka Seibakusha Co., Ltd.) was mixed with conifer sawdust (manufactured by Tomoe Bussan Co., Ltd.) at a dry weight ratio of 2:1 (crushed corn: conifer sawdust) ...

Embodiment 2

[0072] The cultivation step was performed in the same manner as in Example 1 and a cultivation mixture was obtained.

[0073] Next, the mixture was transferred to a germination room, and germination was carried out for 7 days under a light intensity of 50 lux or less (30 minutes between light and dark), wherein the temperature of the germination room was controlled at 16° C. (manufactured by Saginomiya Seisakusho, Inc.) indicates a humidity value of 115% to 120%. At this time, 8 groups of test schemes were set up (12 bottles were used in each test scheme), and germination was carried out for 0 days, 1 day, 2 days, 3 days, 4 days, 5 days or 6 days without removing the bottle caps. , and continue to germinate for 7 days, 6 days, 5 days, 4 days, 3 days, 2 days or 1 day after removing the bottle cap and turning the bottle over, thereby completing the germination step. CO in the germination stage was measured by the same method as in Example 1 2 concentration. CO in the cap 2 T...

Embodiment 3

[0078] The cultivation step was performed in the same manner as in Example 1 and a cultivation mixture was obtained.

[0079] Next, three sets of test protocols were set up for germination. That is, as a control group, after removing the cap and turning the bottle over, germination was carried out for 7 days in a germination chamber in which the temperature of the germination chamber was controlled at 16° C. and the humidity represented by Humid Eye 100 (manufactured by Saginomiya Seisakusho, Inc.) The value is 115% to 120%, and the illuminance on the medium surface is 50 lux or less (30 minutes between light and dark). The remaining two test schemes are as follows: in one scheme, the culture bottle is capped (capping scheme), and in the other scheme as a control, the fixed area between the bottle cap and the culture flask is sealed with polyvinyl chloride tape. The semicircle is partially sealed (semi-sealed solution) and germination takes place in the same germination chamb...

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Abstract

A fungal bed cultivation method of a hon-shimeji mushroom, in which the sprouting step and / or fruit body growing step is carried out under an environmental condition of high CO2 concentration, is provided. Examples of the environmental condition of high CO2 concentration include a CO2 concentration of 2,500 ppm or more in the sprouting step and a CO2 concentration of 5,000 ppm or more in the fruit body growing step. Since the formation ratio of a budlet in the fungal bed cultivation of a hon-shimeji mushroom is improved by embodiments of the present invention, stable production of a hon-shimeji mushroom by its large scale commercial cultivation becomes possible.

Description

[0001] foreign priority [0002] This application is based on Japanese Patent Application No. 2008-304138 filed on November 28, 2008, the entire contents of which are incorporated herein by reference. technical field [0003] The invention relates to a fungal bed cultivation method of hon-shimeji mushroom (Lyophyllumshimeji). Background technique [0004] Honshimeji mushrooms are mushrooms that grow on the ground in white oak forests or mixed forests of white oak and Japanese red pine around mid-October. In Japan, hon shimji mushrooms are considered the highest quality edible mushrooms. Specifically, there is a Japanese proverb that refers to both hon-shampoo mushrooms and matsutake (Tricholoma matsutake): "Tricholoma matsutake is fragrant, and mamsutake is beautiful." In recent years, it has been envisioned to use culture medium to artificially cultivate edible mushrooms (such as Flammulina velutipes, Pleurotus otreatus, Pholiota nameko, Hypsizygus marmoreus, Grifola fron...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G1/04A01G18/00A01G18/40A01G18/69A01G9/24
Inventor 河合高志桥本麻由杉森武日下部克彦喜多昭彦加藤郁之进
Owner TAKARA HOLDINGS
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