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Fungal bed cultivation method of hon-shimeji mushroom

一种栽培方法、菌床的技术，应用在本占地菇的菌床栽培领域，能够解决价格高、培养基成本高、尚未达到工业生产的水平等问题，达到优异形状的效果

Inactive Publication Date: 2013-04-24
TAKARA HOLDINGS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, since the price of wheat used in the medium used in the method of Patent Document 1 (JP-A-07-115844) is high, the cost of the medium is high
In addition, the yield of fruiting bodies formed in the inventor's method of Patent Document 2 (JP-A-06-153695) is low, so it has not yet reached the level of industrial production

Method used

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  • Fungal bed cultivation method of hon-shimeji mushroom
  • Fungal bed cultivation method of hon-shimeji mushroom
  • Fungal bed cultivation method of hon-shimeji mushroom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Inoculate the hyphae of Lyophyllum shimeji La 01-27 (FERM BP-10960) in 100 ml of PGY liquid medium (composition: glucose 2.0% (w / v), peptone 0.2% (w / v), yeast extract 0.2% (w / v), KH 2 PO 4 0.05%(w / v), MgSO 4 ·7H 2 O0.05% (w / v)), cultured at 25°C for 7 days with shaking (100 rpm). 2 ml of the culture mixture was cultured in 200 ml of the same medium, and cultured under shaking (100 rpm) for 7 days. Subsequently, the entire culture mixture was inoculated in a fermenter (manufactured by Komatsukawa Seisakusho Co., Ltd.) with a volume of 200 liters and 160 liters of the same medium, and cultured with stirring for 6 days (stirring speed: 100 rpm, ventilation rate: 25 liters) / Min), thereby preparing a liquid strain culture. On the other hand, crushed corn (produced by Iisaka Seibakusha Co., Ltd.) and coniferous sawdust (produced by Tomoe Bussan Co., Ltd.) were mixed at a dry weight ratio of 2:1 (crushed corn: coniferous sawdust) , And add water in a volume such that the fin...

Embodiment 2

[0072] The cultivation step was completed in the same manner as in Example 1 and the cultivation mixture was obtained.

[0073] Next, the mixture was transferred to the germination chamber and germinated for 7 days under an illuminance of 50 lux or less (the interval between light and darkness was 30 minutes), where the temperature of the germination chamber was controlled to 16°C, and the germination was controlled by Humid Eye 100 (Manufactured by Saginomiya Seisakusho, Inc.) indicates a humidity value of 115% to 120%. At this time, set up 8 groups of test schemes (each test scheme uses 12 bottles), and carry out sprouting for 0 days, 1 day, 2 days, 3 days, 4 days, 5 days or 6 days without removing the caps And after removing the bottle cap and turning over the bottle, continue to germinate for 7 days, 6 days, 5 days, 4 days, 3 days, 2 days or 1 day to complete the germination step. Measure CO in the germination stage by the same method as in Example 1. 2 concentration. CO in...

Embodiment 3

[0078] The cultivation step was completed in the same manner as in Example 1 and the cultivation mixture was obtained.

[0079] Next, set up three sets of test programs for germination. That is, as a control group, after removing the cap and turning over the bottle, germinate in the germination chamber for 7 days, in which the temperature of the germination chamber was controlled to 16°C, and the humidity indicated by Humid Eye 100 (manufactured by Saginomiya Seisakusho, Inc.) The value is 115% to 120%, and the light intensity on the surface of the culture medium is 50 lux or less (the light-dark interval is 30 minutes). The remaining two test schemes are as follows: In one scheme, the culture bottle is capped (capping scheme), and in the other as a control scheme, the fixed area between the cap and the culture bottle is fixed with PVC tape. The semicircle is partially sealed (semi-sealed solution), and germination is carried out in the same germination chamber.

[0080] Measure ...

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Abstract

A fungal bed cultivation method of a hon-shimeji mushroom, in which the sprouting step and / or fruit body growing step is carried out under an environmental condition of high CO2 concentration, is provided. Examples of the environmental condition of high CO2 concentration include a CO2 concentration of 2,500 ppm or more in the sprouting step and a CO2 concentration of 5,000 ppm or more in the fruit body growing step. Since the formation ratio of a budlet in the fungal bed cultivation of a hon-shimeji mushroom is improved by embodiments of the present invention, stable production of a hon-shimeji mushroom by its large scale commercial cultivation becomes possible.

Description

[0001] Foreign priority [0002] This application is based on Japanese Patent Application No. 2008-304138 filed on November 28, 2008, the entire content of which is incorporated herein by reference. Technical field [0003] The present invention relates to a fungus bed cultivation method of hon-shimeji mushroom (Lyophyllumshimeji). Background technique [0004] This shiitake mushroom is a mushroom that grows on the ground of a white oak forest or a mixed forest of white oak and Japanese red pine around mid-October. In Japan, Honshiji mushroom is considered to be the highest quality mushroom among edible mushrooms. Specifically, there is a Japanese proverb that mentions both the tricholoma matsutake and Tricholoma matsutake: "Matsutake is fragrant, and the land is beautiful." In recent years, people have envisaged the use of culture medium to artificially cultivate edible mushrooms (such as Flammulinavelutipes, Pleurotus ostreatus, Pholiota nameko, Hypsizygus marmoreus, Grifola fro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
CPCA01G1/04A01G18/00A01G18/40A01G18/69A01G9/24
Inventor 河合高志桥本麻由杉森武日下部克彦喜多昭彦加藤郁之进
Owner TAKARA HOLDINGS
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