Chinese traditional medicine preparation for reversing multiple medicine resistance of tumor cells and preparation method thereof

Abstract

The invention discloses a Chinese traditional medicine preparation for reversing multiple medicine resistance of tumor cells, which is prepared from the following raw material medicines in parts by weight: 1-2 parts of baicalin and 1-2 parts of jasminoidin. The preparation method comprises the following steps: firstly, preparing the baicalin and the jasminoidin, then taking and mixing the baicalin and the jasminoidin, and adding water for dissolving the mixture to prepare a mixed preparation; or taking and mixing the baicalin and the jasminoidin, adding water for dissolving the mixture, then adding starch or dextrin, carrying out heating concentration or spray drying, and then adding starch prill for preparing a granular preparation or a capsule preparation. The inventors of the application make a large amount of experimental researches, and the research results show that in the invention, the Chinese traditional medicine preparation containing 1-2 parts of baicalin and 1-2 parts of jasminoidin has obvious effect on reducing an expression rate of a medicine resistant S180 tumor cell P170 of a mouse induced by chemotherapy, and the mechanism of the Chinese traditional medicine preparation is possibly related to reduction of the P170 and over expression of TopoII. The Chinese traditional medicine preparation has the functions of inhibiting cell growth of K562 / ADM medicine resistant tumor cells, reversing medicine resistance and promoting cell apoptosis.

Description

technical field [0001] The invention relates to a traditional Chinese medicine preparation for reversing multidrug resistance of tumor cells and a preparation method thereof, belonging to the field of medical biology. Background technique [0002] Multidrug resistance of tumor cells is the main reason for the failure of chemotherapy, and it is also an important factor in the death of cancer patients. Tumor cells can develop multidrug resistance and chemotherapy resistance through various channels. These channels can be briefly summarized as follows: At the cell membrane level, the expression of membrane proteins such as P170, MRP, LRP, BCRP, etc. increases, which accelerates drug efflux and reduces drug accumulation in cells; at the cytoplasmic level, including GST, PKC, P450 reductase, coenzyme II, etc. Enzyme activity in the cytoplasm is enhanced, the detoxification function of the cell is enhanced, and the metabolism and degradation of chemotherapy drugs are accelerated; ...

AI Technical Summary

Problems solved by technology

[0002] Multidrug resistance of tumor cells is the main reason for the failure of chemotherapy, and it is also an important factor in the death of cancer patients. Tumor cells can develop multidrug resistance and chemotherapy resistance through various channels. These channels can be briefly summarized as follows: At the cell membrane level, the expression of membrane proteins such as P170, MRP, LRP, BCRP, etc. increases, which accelerates drug efflux and reduces drug accumulation in cells; at the cytoplasmic level, including GST, PKC, P450 reductase, coenzyme II, etc. Enzyme activity in the cytoplasm is enhanced, the detoxification function of the cell is enhanced, and the metabolism and degradation of chemotherapy drugs are accelerated; at the nuclear level, the number, activity, structure and distribution of TOPO II are changed, and the DNA repair ability is enhanced, which reduces DNA damage; Changes in the expression levels of apoptosis-related genes and even oncogenes in multidrug-resistant tumor cells, such as the increased expression of Bcl-2 and Bcl-xl, reduce tumor cell apoptosis induced by chemotherapeutic drugs and lead to drug resistance. Resistance to chemotherapeutic drugs, causing chemotherapy failure and reducing clinical chemotherapy effect

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