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Magnetic immunochromatographic test strip for detecting hepatitis B e antigen and preparation method thereof

A technology of magnetic immunochromatography and test strips, which is applied in the field of medical testing, can solve problems such as long reaction time, low sensitivity, and the influence of subjective judgment of observers, and achieve the effects of simplifying the preparation process, avoiding subjectivity, and easy operation

Inactive Publication Date: 2010-06-23
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] ELISA and CLIA methods are currently commonly used detection technologies in clinical immunoassays, but the reaction time is relatively long, and both require complex equipment such as a microplate reader or luminometer, a plate washer, and an incubator, which cannot meet the clinical emergency preoperative detection. The requirement to guide medication before rescue also limits the application in some primary medical units
The rapid detection test strip represented by colloidal gold or latex particles has the disadvantage that the result needs to be observed with the naked eye, which is easily affected by the subjective judgment of the observer, and has low sensitivity

Method used

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  • Magnetic immunochromatographic test strip for detecting hepatitis B e antigen and preparation method thereof
  • Magnetic immunochromatographic test strip for detecting hepatitis B e antigen and preparation method thereof

Examples

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Embodiment 1

[0030] Preparation method of magnetic immunochromatography test strip and test paper box for detecting hepatitis B e antigen in blood

[0031] The preparation method of test strip and test paper box of the present embodiment comprises the following steps:

[0032] A. Antibody preparation: select commercial HBeAb, dialyze against 20 mM PBS with pH 7.2 (pH 7.0-7.6 is applicable), and dialyze overnight at 4° C. for later use.

[0033] B. Preparation of coating film:

[0034] Coating buffer preparation: 0.02M phosphate buffer solution (PBS) with pH 7.2 was used as the coating buffer, which was sterilized by 0.22 μm microporous membrane and stored at 4°C for later use, with a validity period of two weeks.

[0035] Preparation of blocking solution: 0.02M phosphate buffered saline (PBS) containing 0.5% BSA at pH7.2 (pH7.0-7.6 is applicable), 0.22μm microporous membrane filter and sterilize, and store at 4°C for later use. Valid for one week.

[0036] Preparation of coating membrane:...

Embodiment 2

[0061] The use method of detection card of the present invention

[0062] 1. Add sample

[0063] Take out the single-serving test card from the packaging box, tear off the aluminum foil packaging, place the test paper card on a flat table, use a micropipette to take 50 μl sample serum into the sample hole on the card, and then add 50 μl chromatography buffer, and wait for the reaction to proceed for 15 minutes.

[0064] 2. Measurement and result output

[0065] Turn on the MICT detector in advance, insert the detection card into the card slot of the detector, run the instrument, the instrument will automatically read the two-dimensional code information on the card and perform measurement, and immediately print the measurement results, and the negative and positive results will be printed in the results show.

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Abstract

The invention relates to a magnetic immunochromatographic test strip for detecting hepatitis B e antigen in blood and a preparation method thereof. The magnetic immunochromatographic test strip introduces the magnetic micro-particle immunochromatographic technology into the hepatitis B e antigen detection, and the test strip is assembled by sequentially mutually sticking a coating film, a magnetic particle pad combined with hepatitis B e antibody, a sample pad and a water-absorbing pad on a substrate in a staggered manner and then covering a transparent plastic sealing film on the upper layer. A hepatitis B e antigen detection line and a quality control line are pre-coated on the coating film. The magnetic immunochromatographic test strip has the advantages of simple operation, high sensitivity, good specificity and the like.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a magnetic immunochromatographic test strip for detecting hepatitis B e antigen and a preparation method thereof. Background technique [0002] Hepatitis B is caused by hepatitis B virus (HBV), an enveloped virus with a partially double-stranded circular DNA genome, belonging to the Hepadnaviridae family. When the virus replicates in liver cells, it can interfere with liver function, and the immune system activates a series of specific responses to fight and eradicate the infectious agent. As a result of pathological damage, the liver becomes inflamed. Severe pathological consequences of persistent HBV infection include chronic liver insufficiency, cirrhosis and hepatocellular carcinoma (HCC). [0003] HBeAg appears at the same time or later with hepatitis B surface antigen positive after hepatitis B virus infection, and the peak period of HBsAg in the blood is also the peak peri...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/576G01N33/543G01N33/532
Inventor 詹先发应希堂胡国茂郑金来唐宝军于尚永
Owner CHEMCLIN DIAGNOSTICS CO LTD
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