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Application of snake venom cytotoxin -CTX1 in the preparation of drugs with analgesic function

A technology of CTX1 and cytotoxin, applied in the field of application of snake venom cytotoxin-CTX1 in the preparation of drugs with analgesic effect

Inactive Publication Date: 2012-05-30
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, whether CTX1 has analgesic function has not been reported so far

Method used

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  • Application of snake venom cytotoxin -CTX1 in the preparation of drugs with analgesic function
  • Application of snake venom cytotoxin -CTX1 in the preparation of drugs with analgesic function
  • Application of snake venom cytotoxin -CTX1 in the preparation of drugs with analgesic function

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] Part I: Preparation of Snake Venom Cytotoxin-CTX1 and Determination of Its Molecular Weight and Amino Acid Sequence

[0021] 1.1 Preparation of snake venom cytotoxin-CTX1

[0022] Zhoushan cobra venom samples were collected from Zhanjiang area, one of the dominant areas of natural and wild distribution of Zhoushan cobra in mainland China, and CTX1 was obtained by the following isolation and purification:

[0023] (1) Gel filtration: Using Sephacryl S-100HR filler, the Zhoushan cobra venom sample was filtered through a molecular sieve through the AKTA explorer 100 rapid purification process development system (preparative high-performance liquid chromatography), and three toxicities I, II, and III were obtained. Components were desalted with G-50 prepacked column HiPrep 26 / 10 Desaltin, freeze-dried and set aside;

[0024] The column size is 26 × 100mm, and the gel column is first equilibrated with two column volumes of 0.05M pH6.4 phosphate buffer solution, and the snak...

Embodiment 1

[0033] In this example, the analgesic effect of CTX1 on visceral pain caused by noxious stimuli in mice was tested by the acetic acid writhing method.

[0034] Animals are injected intraperitoneally with acetic acid solution, which can simulate the symptoms of abdominal visceral pain caused by abdominal inflammation, and is often used for screening new analgesic drugs. By observing the difference in writhing symptoms between the drug treatment group and the control group, it can be determined whether the drug has an analgesic effect and its dose-effect curve.

[0035] Mice (body weight 20-25g, both male and female) were intraperitoneally injected with different doses of CTX1 (experimental group), morphine (positive control group) or normal saline (negative control group). ml 0.6% acetic acid solution. After the treatment with acetic acid solution, the mice were placed in glass beakers for 5 minutes, then observed for 10 minutes, and the number of typical writhing symptoms occ...

Embodiment 2

[0042] In this example, the analgesic effect of CTX1 on somatic pain caused by noxious stimuli and pain after morphine addiction (pain symptom group) in rats was tested by the hot plate method.

[0043] The hot plate method is a method commonly used in the screening and detection of analgesic drugs. It is also a method that can determine the analgesic mechanism of the central nervous system and peripheral nerves. It has a wide range of applications. After mice are stimulated by heat, they can lick their hind feet. or paw withdrawal and other typical somatic pain responses. The length (s) of the latent period of thermal pain response was measured, which was consistent with the change of the animal's pain threshold.

[0044] Take normal and morphine-addicted rats (male, body weight 180-220g) and place them on the metal hot plate (surface temperature: 55±0.5°C) of the UGO pain tester, and start timing until the rats lick their hind feet or shrink back. Paw, stop timing, this per...

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Abstract

The invention discloses application of snake venom cytotoxin-CTX1 in the preparation of drugs with analgesic function, particularly discloses the application of snake venom cytotoxin-CTX1 in the preparation of analgesic drugs for somatic pains caused by noxious stimulation, visceral pains and the pains due to morphine addiction. The cytotoxin-CTX1 has good analgesic function to the somatic pains caused by noxious stimulation, the visceral pains and the pains due to morphine addiction. Moreover snake venom cytotoxin-CTX1 does not cause addiction or tolerance phenomenon after continuous medication for 12 days. Therefore, the snake venom cytotoxin-CTX1 has good prospects for developing into new analgesics.

Description

technical field [0001] The present invention relates to the application of snake venom cytotoxin-CTX1 in the preparation of drugs with analgesic effect, in particular to the application of snake venom cytotoxin-CTX1 in the preparation of body pain and visceral pain caused by noxious stimuli and pain after morphine addiction. application in medicine. Background technique [0002] Snake venom is a huge treasure house of biological resources. Since 1968, British scientists first successfully developed ARIN, a snake venom preparation for the treatment of vascular occlusive diseases, and soon after it was put on the market, it gained huge economic returns. In the past ten years, with the emergence of as many as dozens of snake venom drugs, and achieved good clinical curative effect and sales performance, the development of cheap and high-quality specific drugs from snake venom is becoming a popular choice for biologists, pharmacists and experts from all over the world. A new ho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61P29/00C07K14/46C07K1/36C07K1/18C07K1/16
Inventor 刘先国孔天翰信文君仲维高崔跃董伟华黄绍崔超伟唐娅
Owner SUN YAT SEN UNIV
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