Culture medium for rapid detection of beer anaerobic bacteria, and preparation method thereof

A technology of culture medium and anaerobic bacteria, applied in the field of beer, can solve the problems of small colonies, unfavorable observation, counting, and unsatisfactory results

Active Publication Date: 2012-04-18
TSINGTAO BREWERY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The currently used medium takes 7 days to cultivate harmful anaerobic bacteria in beer, and for Pediococcus.spp and two Lactobacillus lindneri and Lactobacillus paracollinoides with com

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  • Culture medium for rapid detection of beer anaerobic bacteria, and preparation method thereof

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Embodiment 1

[0041] A culture medium for rapid detection of harmful anaerobic bacteria in beer. Its liquid formula is as follows: 1L dosage (g): fructose (fructose) 7.5g, L-cysteine ​​hydrochloride (L-Cysteine ​​HCL) 1.25g , ammonium citrate (ammonium citrate) 1g, beef extract (beef extract) 6.6-6.7g, dextrose (dextrose) 10.3-10.4g, dipotassium phosphate (dipotassium phosphate) 1.3-1.4g, magnesium sulfate (magnesium sulfate) sulfate) 0.05g, manganese sulfate 0.025g, Tween 80 (polysorbate80) 0.6g, proteose peptone No3 5g, sodium acetate 3.3-3.4g, yeast extract (Yeast Extract) 3.3-3.4g, 1.5g of liver concentrate, 0.4g of L-malic acid, 22.5g of maltose, 3.3-3.4g of lactose, tomato juice ) 6g, special peptone (peptonespecial) 12.5g, cytosine (cytidine) 0.1g, deoxythymidine (thymidine) 0.2g.

[0042] A culture medium for rapid detection of harmful anaerobic bacteria in beer, its solid formula is as follows: the amount (g) added in 1 L: add 15 g of agar (Agar) to the above liquid formula.

[0...

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Abstract

The invention provides a culture medium for rapid detection of harmful anaerobic bacteria in beer, which is characterized in that a formulation of 1 liter of liquid is as follows: 7.5 grams of fructose, 1.25 grams of L-cysteine hydrochloride, 1 gram of ammonium citrate, 6.6 to 6.7 grams of beef extract, 10.3 to 10.4 grams of dextroglucose, 1.3 to 1.4 grams of dipotassium phosphate, 0.05 gram of magnesium sulfate, 0.025 gram of manganese sulphate, 0.6 grams of Tween-80, 5 grams of element-peptone, 3.3 to 3.4 grams of sodium acetate, 3.3 to 3.4 grams of yeast extract, 1.5 grams of bovine liver extract powder, 0.4 gram of L-malic acid, 22.5 grams of maltose, 3.3 to 3.4 grams of lactose, 6 grams of tomato juice, 12.5 grams of special peptone, 0.1 gram of cytidine and 0.2 gram of deoxythymine. A solid formulation adds 15 grams of agar to the liquid formulation. The rapid culture medium for harmful beer anaerobic bacteria is prepared by subjecting inorganic nutritional components, organic nutritional components, carbohydrates, growth stimulants, natural extracts, surfactants to the steps of mixing, sub-packaging, sterilization and the like, and has the advantages of higher culture speed and better culture effect.

Description

technical field [0001] The invention belongs to the technical field of beer, and more specifically relates to a culture medium for rapidly detecting harmful anaerobic bacteria in beer in the beer production process and a preparation method thereof. Background technique [0002] Beer brewing is based on the use of brewer's yeast to ferment and control the pollution of harmful microorganisms. What we need is pure fermentation in which only cultured yeasts participate, but during the entire beer brewing process, many factors such as brewing equipment, air, and brewing water may cause harmful microorganisms to enter the wort or fermentation liquid. If harmful microorganisms grow in large numbers, they can contaminate the wort and beer. Contaminated bacteria can cause beer rancidity, peculiar smell, stickiness and turbidity, seriously affect the quality of beer, and cause harm and loss to consumers and beer manufacturers. [0003] Beer is a nutrient-poor, even harsh, medium for...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 陈璐董建军单连菊钱中华杨梅郝俊光
Owner TSINGTAO BREWERY
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