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Novel method for preparation of chitosan nano carrier and functionalization thereof

A chitosan nano and chitosan technology is applied in the field of preparation of new chitosan nano carriers, which can solve the problems of difficulty in modification of degradable materials, lack of reactive groups, etc., achieve good mechanical strength, increase residence time, particle size easy effect

Inactive Publication Date: 2012-07-04
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, general synthetic materials lack sufficient reactive groups, so it is very difficult to modify some widely used degradable materials.

Method used

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  • Novel method for preparation of chitosan nano carrier and functionalization thereof
  • Novel method for preparation of chitosan nano carrier and functionalization thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 1 g of chitosan with a molecular weight of 300,000 and a degree of deacetylation of 95%, and dissolve it in 500 ml of acetic acid buffer solution with a pH of 5.1 to obtain a chitosan solution. Take 50ml of this chitosan solution, under vigorous stirring, slowly add 12.5ml, 2mg / ml sodium tripolyphosphate solution to obtain ion-crosslinked chitosan nanoparticle solution; take 3 bottles of prepared ion-crosslinked shell Add 0.294, 0.588, 0.882ml of 5% glutaraldehyde aqueous solution to the polysaccharide nanoparticle solution, crosslink at 37°C for 12 hours, and then reduce it with excess sodium borohydride to finally prepare nanoparticles with different chemical crosslinking degrees.

[0021] figure 1 Scanning pictures of nanoparticles formed by the 300,000 chitosan prepared in Example 1 under the action of different glutaraldehyde are given. exist figure 1 Among them, picture a is 0.294ml, picture b is 0.588ml, picture c is 0.882ml;

Embodiment 2

[0023] Take 1 g of chitosan with a molecular weight of 150,000 and a degree of deacetylation of 95%, and dissolve it in 500 ml of acetic acid buffer solution with a pH of 5.0 to obtain a chitosan solution. Get 50ml of this chitosan solution, under vigorous stirring, slowly add 15ml, 2mg / ml sodium tripolyphosphate solution, obtain the ion-crosslinked chitosan nanoparticle solution; take the prepared ion-crosslinked chitosan nanoparticle solution Add 0.588ml of 5% glutaraldehyde aqueous solution to the particle solution, cross-link for 10 hours at 37°C, and then reduce with excess sodium borohydride to obtain chitosan nanoparticles with a hydration radius of 340 nm.

Embodiment 3

[0025] Take 1.5 g of chitosan with a molecular weight of 70,000 and a degree of deacetylation of 95%, and dissolve it in 500 ml of acetic acid buffer solution with a pH of 4.9 to obtain a chitosan solution. Get 50ml of this chitosan solution, under vigorous stirring, slowly add 15ml, 2mg / ml sodium tripolyphosphate solution, obtain the ion-crosslinked chitosan nanoparticle solution; take the prepared ion-crosslinked chitosan nanoparticle solution Add 0.882 ml of 5% glutaraldehyde aqueous solution to the particle solution, cross-link at 37° C. for 12 hours, and then reduce with excess sodium borohydride to finally prepare chitosan nanoparticles with a hydration radius of 240 nm.

[0026] Folate Modification of Nanoparticles:

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Abstract

The invention discloses a novel method for preparation of a chitosan nano carrier and functionalization thereof, which relates to the chitosan nano carrier. The invention provides a novel method for preparation of the chitosan nano carrier and functionalization thereof. The chitosan is dissolved in acetic acid solution, meanwhile, pH is adjusted to 4.5-5.5 with NaOH solution, STPP (sodium tripolyphosphate) solution is added to the chitosan solution to obtain nanogels, then, crosslinking glutaraldehyde is provided for the chitosan, centrifugation is conducted after ending of reaction, reduction reaction is conducted with excess sodium borohydride, then centrifugation is conducted again, then, the obtained compound is dispersed in hydrochloric acid solution to remove the unreacted sodium borohydride, then dialysis is conducted, in order to wash STPP to obtain nanoparticles; the folic acid is weighed to be dissolved in the phosphate buffer solution, then the solution is added to chitosannanoparticles water solution, after adding EDCI to the solution, folic acid modified nanoparticles are obtained through reaction; PEG succinimidyl propionate is weighed to be dissolved in the phosphate buffer solution, then is added to the chitosan nanoparticles water solution, after reaction, PEG modified nanoparticles are obtained.

Description

technical field [0001] The invention relates to a chitosan nano-carrier, in particular to a method for preparing and functionalizing a novel chitosan nano-carrier. Background technique [0002] With the development of drug sustained and controlled release technology, some new sustained and controlled drug release technologies continue to emerge. There are a large number of reports on passive targeting by controlling particle size and active targeting by modifying drug carriers with small molecules. Traditional synthetic degradable materials are generally difficult to modify bioactive molecules due to their molecular structure and solubility characteristics, which limits their use, and most natural materials are difficult to achieve the encapsulation of some drugs without modification. Chitosan is a natural biomaterial with good biocompatibility and has a wide range of uses. Most of the previous research mainly focused on preparing suitable carriers by modifying chitosan to w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36
Inventor 张其清邱发杰胡权罗颖侯振清
Owner XIAMEN UNIV
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