Galactooligosaccharides economic purification method

A technology of galactooligosaccharides and purification methods, which is applied in the field of low-cost activated carbon as a chromatographic purification medium, can solve the problems of lack of production methods for separation and purification of galactooligosaccharides, and achieve the effects of high purity, easy realization, and energy saving

Inactive Publication Date: 2010-07-21
YANTAI UNIV
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Problems solved by technology

The industry lacks the production method of separating and purifying galacto-oligosaccharides

Method used

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  • Galactooligosaccharides economic purification method
  • Galactooligosaccharides economic purification method

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[0014] The following is an example of this patent.

[0015] (1) Pretreatment of activated carbon: take an appropriate amount of activated carbon according to the volume of the chromatographic column, wash it with clear water several times, place it in a 1000ml beaker, add 5 times the volume of acetic acid solution (mass fraction 20%), and place it on the electric furnace Heat and boil for 0.5h. After acidification, rinse with distilled water until neutral and set aside. Before packing the activated carbon into the column, heat and boil for 15 minutes to drive out the air bubbles.

[0016] (2) Column packing and equilibration: Pour activated carbon into a 1×100 cm chromatographic column, equilibrate the chromatographic column with distilled water of 3 times the column volume, and the flow rate is 40 ml / h.

[0017] (3) Sample loading and separation: open the valve at the lower end of the column, and close the valve when the water in the upper layer of the column falls to the i...

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Abstract

The invention relates to a galactooligosaccharides economic purification method, in particular to high purity galactooligosaccharides product separated from galactooligosaccharides mixture prepared by enzymatic synthesis by adopting low-cost active carbon as chromatography purification medium. The invention is characterized in that: galactooligosaccharides mixture solution prepared by enzymatic synthesis is added into active carbon chromatography column, organic solvent aqueous solution in different concentrations are used for elution, elution fractions in different stages are collected, firstly membrane method is adopted to concentrate and recycle organic solvent aqueous solution, the organic solvent aqueous solution is returned to chromatography system to be repeatedly used as eluant, lactose concentrated solution is returned to enzymatic synthesis working stage to be recycled as raw material, galactooligosaccharides and glucose concentrated solution is further concentrated by adopting vacuum concentration method, and the concentrated solution is subject to spray drying, thus obtaining high purity galactooligosaccharides product and glucose byproduct.

Description

technical field [0001] The invention relates to a production method for separating and purifying high-purity galacto-oligosaccharides from a mixture of galacto-oligosaccharides synthesized by an enzymatic method, which belongs to the technical field of biochemical industry and involves using cheap activated carbon as a chromatographic purification medium, and the separated galacto-oligosaccharides Lactose can be returned to the enzymatic synthesis process as a raw material for recycling. Background technique [0002] Galacto-oligosaccharides can effectively promote the growth and reproduction of bifidobacteria in the body, improve lipid metabolism, promote calcium absorption and vitamin synthesis, and have the functions of decomposing carcinogens and improving human immunity. As a functional food, galacto-oligosaccharides are mainly used in daily foods such as infant food, low-calorie food, special food for diabetics, jam, bread, pastry, and ice cream. [0003] At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H3/06C07H1/06C07H3/02
Inventor 常秀莲冯咏梅于贞
Owner YANTAI UNIV
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