Ethionine resistance Candida utilis and application thereof

A technology of Candida utilis and ethionine, applied in fermentation, biochemical equipment and methods, microorganisms, etc., to achieve high yield, strong protein synthesis ability, and broad growth nutrition spectrum
CN101781625BInactive Publication Date: 2012-04-04SUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SUZHOU UNIV
Publication Date
2012-04-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an ethionine resistance Candida utilis and application thereof, wherein the Candida utilis SUGS-01 is preserved in China Center for Type Culture Collection (CCTCC) on December 9th, 2009, and the preservation number is CCTCC M 209298. The Candida utilis SUGS-01 is applied to produce SAM and GSH by co-production and fermentation, and the application comprises the following steps: taking the Candida utilis SUGS-01 as the original strain, and after slant culturing and seed culturing, fermenting for at least 24 hours; one litre of fermentation medium contains 35-36g of canesugar, 10-12g of ammonium sulfate, 12-13g of monopotassium phosphate, 0.05-0.06g of magnesium sulfate and 4-5g of L-methionine; and the pH value of the fermentation medium is 5.5-6.0. Because adopting the combined action of UV mutagenesis and gamma-ray mutagenesis, the invention obtains the Candida utilis mutant strain which can co-produce SAM and GSH, and the obtained Candida utilis is used as production strains, has the advantages of wide growth nutrition spectrum, less production of ethanol during the fermentation process, strong capability to synthesize protein and the like, and has good industrial application prospect.
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Description

Technical field

[0001] The invention belongs to microbial fermentation technology, and specifically relates to an ethionine-resistant Candida utilis and its application in the co-production and fermentation of S-adenosylmethionine and glutathione. Background technique

[0002] S-adenosylmethionine (SAM) is an important small molecule substance widely found in biological cells, discovered by Cantoni in 1953. Studies have found that SAM, as a group provider and enzyme inducer, participates in many key biochemical reactions, such as the methylation of lipids, proteins, nucleic acids, transsulfurization reactions, and polyamine synthesis. The physiological functions of SAM in cells involve: methyl donor in methyl reaction; production of methylthioadenosine and homoserine; donor of aminobutyl of tRNA; donor of amino acid chain in the synthesis of vitamin H; gland Donor of glycoside part; inhibition of cytolysin-2,3-amino allosteric enzyme, threonine synthase, pyruvate formate lyase, ...

Claims

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