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Poultry oil emulsion vaccine and preparation method thereof

A technology of oil emulsion and vaccine, which is applied in the field of poultry oil emulsion vaccine and its preparation, can solve problems such as difficulties and achieve the effects of reducing retention, strong ability to induce antibody production, and reducing vaccine viscosity

Inactive Publication Date: 2010-09-15
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially for inactivated vaccines, it is very difficult to prepare a vaccine that can induce sufficient immune protection over a long period of time and has good reproducibility

Method used

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  • Poultry oil emulsion vaccine and preparation method thereof
  • Poultry oil emulsion vaccine and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Dilute 4 parts of Tween-80 with 3 times of water, then autoclave at 121°C for 30 minutes and cool to room temperature; take 60 parts of Newcastle disease antigen with HA titer of 8log2 and 12 parts of 3 times of water to dilute and heat at 121°C Sterilize with Tween-80 for 30 minutes, add 28 parts of water and mix evenly to form the water phase.

[0033] 2. First mix 88 parts of white oil, 2 parts of propylene glycol, 2 parts of lecithin and 2 parts of isomannitol monooleate as an adjuvant, and then mix with 6 parts of Span-80 to make an oil The phase was sterilized at 121°C for 30 minutes, and cooled to room temperature for later use.

[0034] 3. According to the volume ratio of oil phase: water phase = 2:1, first pour the oil phase into the emulsification tank, the stirring speed is 11000rpm, and the speed of adding the water phase to the oil phase is 0.6mL / s, and then continue to emulsify at a speed of 15500rpm for 5min, and divide the prepared vaccine into thre...

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PUM

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Abstract

Te invention discloses a poultry oil emulsion vaccine and a preparation method thereof, which is a water-in-oil adjuvant vaccine comprising 33.3 volume percent of water phase and 66.7 volume percent of oil phase, wherein the water phase contains an effective dose of antigen which is biologically acceptable and a hydrophilic nonionic surfactant; and the oil phase contains mineral oil, lecithin, propylene glycol, isomannino monooleate and a lipophilic nonionic surfactant. The vaccine does not contain aluminium stearate, can keep high antibody producing capacity for a long time and solve the problem of animal stress caused by vaccine injection and vaccine residue.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a poultry oil emulsion vaccine and a preparation method thereof. Background technique [0002] my country's poultry meat products have been repeatedly sealed off. From August 1, 1996 to May 24, 2001, the European Union closed my country's customs for five years on the grounds that my country's poultry meat exports contained animal diseases, pesticides, and veterinary drug residues. In 2004, the European Union completely banned the import of poultry products from my country on the grounds of bird flu, and it was not until August 2008 that the ban on poultry products from my country was lifted. Just lifted the ban and encountered the "quota" problem. The prerequisite for solving all problems is to improve the quality of poultry products in our country. In order to reduce the occurrence of poultry diseases, and at the same time avoid the negative...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/145A61K39/17A61K39/215A61K39/12A61K9/107A61P31/16A61P31/12A61K47/10A61K47/24A61K47/26
Inventor 张许科孙进忠乔荣岑陶家权张海洋习向锋李三
Owner PU LIKE BIO ENG
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