Klebsiella pneumoniae for inducing soybean to resist atrazine and application
A technology of atrazine and soybeans, which is applied in the field of inducing Klebsiella pneumoniae resistant to atrazine in soybeans, and can solve the problems of difficulty in weeding, high cost of herbicides, and easy occurrence of phytotoxicity by herbicides
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Embodiment 1
[0019] Embodiment 1: the acquisition of Klebsiella pneumoniae (Klebsiella penumoniae) Sneb YK
[0020] Take 1g of soil from a soybean field, add 100ml of sterile water, take 200μl, add 20ml of sterilized NA liquid medium cooled to below 35°C, cultivate at 25°C and wait for colonies to grow. The colonies of Klebsiella pneumoniae (Klebsiella penumoniae) were preserved separately for multiplication and physiological and biochemical assays, and the culture properties conformed to the characteristics of Klebsiella penumoniae (Klebsiella penumoniae) and were preserved for future use.
Embodiment 2
[0021] Embodiment 2: the cultivation of Klebsiella pneumoniae (Klebsiella penumoniae) Sneb YK
[0022] First, Klebsiella penumoniae (Klebsiella penumoniae) Sneb YK strain was fermented and cultured, and then the metabolites after fermentation and cultured were tested for soybean-inducing activity to determine their inducing effect on soybean.
[0023] Klebsiella penumoniae (Klebsiella penumoniae) Sneb YK thalline is inoculated on the test tube agar medium, and the medium formula is NA medium, namely ingredients: beef extract 3g, peptone 10g, yeast extract 1g, sucrose 10g , agar 20g, add water to 1000mL. Incubate at 25°C for 10 days to obtain test tube species.
[0024] The test tube seed is inoculated into the 250mL triangular flask (100mL per bottle) liquid medium again, and the medium formula is: by weight percentage, containing beef extract 0.2%, peptone 1%, yeast extract 0.5%, sucrose 1%, The remainder was distilled water with a pH of 8. Cultivate at 25°C, shaker speed ...
Embodiment 3
[0025] Embodiment 3: the fermentation of Klebsiella pneumoniae (Klebsiella penumoniae) Sneb YK
[0026] It is basically the same as in Example 1, except that the liquid-cultured strains are inoculated into a 1000 L fermenter for fermentation, cultured at 25° C., pH is 8, and fermented for 48 hours.
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