Klebsiella pneumoniae for inducing soybean to resist atrazine and application

A technology of atrazine and soybeans, which is applied in the field of inducing Klebsiella pneumoniae resistant to atrazine in soybeans, and can solve the problems of difficulty in weeding, high cost of herbicides, and easy occurrence of phytotoxicity by herbicides

Active Publication Date: 2010-09-15
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For soybean fields, weeding is more difficult, and the cost of herbicides in soybean fields is relatively high, and the use of herbicides is prone to phytotoxicity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the acquisition of Klebsiella pneumoniae (Klebsiella penumoniae) Sneb YK

[0020] Take 1g of soil from a soybean field, add 100ml of sterile water, take 200μl, add 20ml of sterilized NA liquid medium cooled to below 35°C, cultivate at 25°C and wait for colonies to grow. The colonies of Klebsiella pneumoniae (Klebsiella penumoniae) were preserved separately for multiplication and physiological and biochemical assays, and the culture properties conformed to the characteristics of Klebsiella penumoniae (Klebsiella penumoniae) and were preserved for future use.

Embodiment 2

[0021] Embodiment 2: the cultivation of Klebsiella pneumoniae (Klebsiella penumoniae) Sneb YK

[0022] First, Klebsiella penumoniae (Klebsiella penumoniae) Sneb YK strain was fermented and cultured, and then the metabolites after fermentation and cultured were tested for soybean-inducing activity to determine their inducing effect on soybean.

[0023] Klebsiella penumoniae (Klebsiella penumoniae) Sneb YK thalline is inoculated on the test tube agar medium, and the medium formula is NA medium, namely ingredients: beef extract 3g, peptone 10g, yeast extract 1g, sucrose 10g , agar 20g, add water to 1000mL. Incubate at 25°C for 10 days to obtain test tube species.

[0024] The test tube seed is inoculated into the 250mL triangular flask (100mL per bottle) liquid medium again, and the medium formula is: by weight percentage, containing beef extract 0.2%, peptone 1%, yeast extract 0.5%, sucrose 1%, The remainder was distilled water with a pH of 8. Cultivate at 25°C, shaker speed ...

Embodiment 3

[0025] Embodiment 3: the fermentation of Klebsiella pneumoniae (Klebsiella penumoniae) Sneb YK

[0026] It is basically the same as in Example 1, except that the liquid-cultured strains are inoculated into a 1000 L fermenter for fermentation, cultured at 25° C., pH is 8, and fermented for 48 hours.

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PUM

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Abstract

The invention relates to a culture method of Klebsiella pneumoniae SnebYK for inducing soybean to generate resistance to atrazine after the treatment of soybean seeds and application thereof, belonging to the technical field of microorganism agricultural pesticide. The invention relates to a bacteria strain SnebYK, which is preserved in China committee for culture preservation of general microorganism centre with the preservation number of CGMCC No.3471 in NOV.24th, 2009. The soybean seeds are treated by a metabolin through culture preparation of liquid fermentation, the soybeans can generate the resistance to atrazine when germinating. The soybean treated by the metabolin of the bacteria can be interbred with the grass family crops including corns and the like, and a uniform atrazine weed killer is adopted to weed out, thereby the difficulty of weeding out the grasses intercropping between the corns and the soybeans is solved, and the Klebsiella pneumoniae has favorable application development prospect.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to Klebsiella pneumoniae for inducing soybean resistance to atrazine and its application. Background technique: [0002] Atrazine is a dry field herbicide widely used in the world, and its Chinese product name is atrazine. Atrazine (Atrazine), chemical name 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine, is a selective triazine herbicide, introduced in 1952 by Switzerland Ciba- Developed by Geigy Chemical Company. It can be used to control annual grass weeds and broadleaf weeds in corn, sorghum, sugar cane, fruit trees, nurseries, woodlands, etc. Due to its excellent weeding efficacy and low price, it was put into commercial production in 1959. It can be mixed with various amide herbicides such as acetamine, alachlor, and methamphetamine to enhance the herbicidal activity and application range. Among the dryland herbicides, atrazine plays an impo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/22
Inventor 段玉玺罗在全陈立杰王媛媛朱晓峰
Owner SHENYANG AGRI UNIV
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