Substrate of traditional Chinese medicine biochip as well as preparation method and use thereof

A biochip and traditional Chinese medicine technology, applied in the fields of biology and chemistry, can solve the problems of long screening period, no report on the substrate for immobilizing small organic molecules, and low efficiency, and achieve the effect of high-throughput screening

Inactive Publication Date: 2010-09-15
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of screening is to screen and match drugs from the mechanism, and it is not a system for targeted screening of active ingredients of drugs. It has the disadvantages of long screening cycle, high cost, and low efficiency.
The active ingredients of Chinese herbal medicine are mostly small molecular organic compounds such as aldehydes, ketones, glycosides, and alkaloids. However, the development of currently commercially available biochip substrates is mainly aimed at immobilizing biological macromolecules such as proteins and nucleic acids. The base of small organic molecules has not been reported

Method used

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  • Substrate of traditional Chinese medicine biochip as well as preparation method and use thereof
  • Substrate of traditional Chinese medicine biochip as well as preparation method and use thereof
  • Substrate of traditional Chinese medicine biochip as well as preparation method and use thereof

Examples

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Effect test

example 1

[0025] One, the preparation of Chinese medicine biochip substrate of the present invention

[0026] 1. Take the slide, soak it in 1M sodium hydroxide solution for 2 hours, clean it with sterile water, then soak it in dilute hydrochloric acid with a volume percentage of 5% overnight, and finally wash it with sterile water;

[0027] 2. Soak the cleaned glass slide in 95% ethanol solution containing 10% glycidyl-propoxytrimethoxysilane by volume for 1 hour, then put it into 0.3M hydrochloric acid for 1.5 hours, and then Rinse 3 times with sterilized water and dry in air;

[0028] 3. React the silanized glass slide in the activation solution for 1 hour, and keep shaking in the fume hood during the reaction. After the reaction, wash with acetone 3 times.

[0029] The activation solution in step 3 was prepared as follows: take 10ml of divinyl sulfone and dissolve it in 100ml of 0.5mol / L sodium carbonate solution.

[0030] 2. Preparation of traditional Chinese medicine biochip

...

example 2

[0047] Preparation of traditional Chinese medicine biochip substrate of the present invention

[0048] 1. Take the slide, soak it in 1M sodium hydroxide solution for 3 hours, wash it with sterile water, and then place it in dilute H with a volume percentage of 3%. 2 SO 4 Soak overnight and finally wash with sterile water;

[0049] 2. Soak the cleaned glass slide in 95% ethanol solution containing 15% glycidyl-propoxytrimethoxysilane by volume for 1.5 hours, then hydrolyze it in 0.3M sulfuric acid for 1 hour, and then Rinse 3 times with sterilized water and dry in air;

[0050] 3. React the silanized slides in the activation solution for 2 hours, and keep shaking in the fume hood during the reaction. After the reaction, wash with acetone 3 times.

[0051] The activation solution in step 3 was prepared as follows: take 10ml of divinyl sulfone and dissolve it in 100ml of 0.5mol / L sodium carbonate solution.

example 3

[0053] Preparation of traditional Chinese medicine biochip substrate of the present invention

[0054] 1. Take the slide, soak it in 2M potassium hydroxide solution for 3 hours, clean it with sterile water, then soak it in dilute HCl with a volume percentage of 10% overnight, and finally wash it with sterile water;

[0055] 2. Soak the cleaned glass slide in 95% ethanol solution containing 10% glycidyl-propoxytrimethoxysilane by volume for 2 hours, then put it into 0.4M hydrochloric acid for 1 hour, and then Rinse 3 times with sterilized water and dry in air;

[0056] 3. React the silanized glass slide in the activation solution for 1.5 hours, and keep shaking in the fume hood during the reaction. After the reaction, wash with acetone 3 times.

[0057] The activation solution in step 3 was prepared as follows: take 10ml of divinyl sulfone and dissolve it in 100ml of 0.5mol / L sodium carbonate solution.

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Abstract

The invention provides a substrate of a traditional Chinese medicine biochip formed by a silanizated slide and an organic active group connected through -O-. The substrate of the traditional Chinese medicine biochip is characterized in that the organic active group is -(CH2)2SO2CHCH2, an active ethenyl is at the end of the group and can be in cross-linking reaction with micromolecular organic matters such as carbonyls, glycosides and alkaloids and the like so that the organic micromolecule can be fixed on the substrate to prepare the traditional Chinese medicine biochip using the micromolecular organic matters as a probe, for example, a high throughput biochip which screens active ingredients of Chinese medicinal herb.

Description

technical field [0001] The present invention relates to the fields of biology and chemistry. Background technique [0002] A biochip refers to a microarray of biological information molecules immobilized on a stationary phase support medium at high density, and each molecule and its position in the array are known. Biochip technology originated from the hybridization of nucleic acid molecules, the so-called gene chip, which can detect the genome and its expression of organisms in real time. After more than ten years of development, biochips have developed in a diversified direction, realizing accurate, rapid and large-scale detection of biological components such as compounds, proteins, peptides, DNA, and biological tissues. [0003] A biochip consists of two parts: a substrate and a probe. The substrate is generally a glass sheet, a silicon sheet or a nylon film modified by an organic active group. Probes are substances that can specifically bind to biological components...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
Inventor 吴清华张宝马文丽沈群郑文岭
Owner SOUTHERN MEDICAL UNIVERSITY
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