One-step RT-PCR (Reverse Transcription Polymerase Chain Reaction) detection reagent kit for Cucumber green mottle mosaic virus and detection method thereof
An RT-PCR and detection kit technology, applied in the field of one-step RT-PCR detection kits for cucumber green mottle mosaic virus, can solve the problems of low reliability, long detection period, complicated processing, etc. The effect of reducing detection cost and simple production method
Inactive Publication Date: 2010-09-29
INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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The invention relates to a one-step RT-PCR (Reverse Transcription Polymerase Chain Reaction) detection reagent kit for Cucumber green mottle mosaic virus and a detection method thereof. The reagent kit comprises the following various agents for a one-step RT-PRC reaction: an upstream primer of Cucumber green mottle mosaic virus, a downstream primer of Cucumber green mottle mosaic virus, a PCR Buffer, Mgc12, dNTPs, an RNA enzyme inhibitor, a reverse transcriptase, a DNA polymerase, positive control containing Cucumber green mottle mosaic virus and negative control containing no Cucumber green mottle mosaic virus and RNase-free ddH2O. The invention has the advantages of simple preparation method and convenient large-scale industrialized production, and the detection method has the advantages of simple and convenient operation, high sensitivity, strong specificity and good accuracy. The whole detection process is accomplished in a PCR tube and suitable for rapid detection of a batch of samples, thereby simplifying the operation procedure, reducing the detection time, avoiding the cross contamination, and improving the detection efficiency.
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