Bacteriaemia aspartame assay kit and assay method thereof

Abstract

The invention relates to the field of clinical microorganism identification, and provides a kit for rapidly identifying a bacterium in a blood source and a method thereof. The kit comprises: two universal primers amplify the V1 variable region of a bacterium 16S r RNA, one sequencing primer measures the sequence of a V1 forward sector, HRP-avidin labels a magnetic bead, and genus is judged by comparing sequencing results and a database. The kit can sequence and distinguish the bacterium in the blood source at a time, can be used for clinical diagnosis of bacteriaemia and ichorrhemia, not only saves the valuable rescuing time for clinical diagnosis and treatment, and has low cost, convenient operation and strong specificity.

Description

technical field [0001] The invention belongs to the technical field of clinical microorganism identification, and in particular relates to a kit and a detection method for rapidly identifying pathogenic bacteria in blood samples. Background technique [0002] Bacteremia in adults and children is a disease with high morbidity and mortality worldwide. With 20 million cases of bacteremia worldwide each year, bacteremia remains a major challenge even in medical practice in developed countries, especially in neonatal intensive care units, transplant wards, hospitalized patients, immunosuppressed and postoperative patients 135,000 deaths per year in Europe and 215,000 in the United States. In order to achieve a better therapeutic effect, targeted antibiotics should be used within 6 hours of symptom onset. Early identification of bacteria is especially important for immunosuppressed patients, who have a wider range of bacteria infections than ordinary patients and are more unpred...

AI Technical Summary

Problems solved by technology

Low sensitivity due to limitations such as insufficient blood volume, the presence of antibiotics in the blood, and the need for special nutrition is also a limiting factor for traditional culture

In addition, traditional identification methods require Gram staining, biochemical reaction identification, etc. after obtaining positive results, and there are many potential problems in this: ①Low-specific pathogens need to be tested; ②Multiple biochemical tests are often required Simultaneously; ③ separation and purification before biochemical identification, at least 1-3 days; ④ phenotypic test cannot be repeated

Inappropriate use of antibiotics often leads to treatment failure, resulting in prolonged hospitalization, complications, drug resistance, and increased testing costs

Images