Method for producing marasmius androsaceus fermented powder by adopting optimized solid culture medium

A technology of solid medium and Anluotong bacteria powder, which is applied in the direction of botany equipment and methods, applications, non-central analgesics, etc., and can solve the problems of long production time, low yield of mycelium, high manufacturing cost, etc.

Inactive Publication Date: 2010-11-10
漯河市方汇药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid culture medium takes a long time to cultivate and the mycelium yie

Method used

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  • Method for producing marasmius androsaceus fermented powder by adopting optimized solid culture medium
  • Method for producing marasmius androsaceus fermented powder by adopting optimized solid culture medium

Examples

Experimental program
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Embodiment 1

[0021] Embodiment 1: adopt the method for solid culture medium to produce Anluotong bacterial powder, comprise the following steps: 1.. Sterilization: take by weighing 50kg of wheat bran, 20kg of rice hulls, oral glucose 5kg, potassium dihydrogen phosphate 0.05kg, magnesium sulfate 0.025 kg, add water to 100kg, pack into bags, and sterilize at 118°C for 2 hours; ②. Inoculation culture: inoculate at room temperature; ③. Cultivation: culture at 23°C for 45 days, dry at 55°C for 12 hours to obtain P. and its culture medium powder; ④. Extraction: add 95% ethanol to extract twice, add 4 times the weight of ethanol for the first time, boil for 1.5h, add 2 times the amount of ethanol for the second time, boil for 1h; ⑤ . Concentration: Combine the extracts, concentrate to a water content of 20%, recover ethanol, and obtain the ethanol extract of Dermatobacter anluo and its culture medium; Anluotong fungus powder, wherein the ethanol extract of Dermatobacter anluo and its medium: Derm...

Embodiment 2

[0022] Embodiment 2: Step ① solid medium in this embodiment takes the following raw materials: take by weighing 51kg of wheat bran, 21kg of rice husk, 6kg of oral glucose, 0.06kg of potassium dihydrogen phosphate, 0.03kg of magnesium sulfate, add water to 100kg, pack into bags, Sterilized at 118°C for 2 hours; Step 3. Cultivation temperature and time are the same as in Example 1 to prepare Dermatophagus anluo and its culture medium powder with a water content of 3.5%; Step 5. Combine the extracts, concentrate to a water content of 22%, and recover Ethanol, the ethanol extract of Dermatomella parvula and its culture medium; other steps are the same as in Example 1.

Embodiment 3

[0023] Embodiment 3: Step ① solid medium in this embodiment takes the following raw materials: take 55kg of wheat bran, 23kg of rice husks, take 8kg of glucose, 0.08kg of potassium dihydrogen phosphate, and 0.04kg of magnesium sulfate, add water to 100kg, pack into bags, Sterilize at 118°C for 2 hours; Step ③Cultivate at 23°C for 45 days, dry at 55°C for 12 hours to obtain Dermatophaga anluo and its culture medium powder with a water content of 4%; Step ⑤Combine the extracts, concentrate to a water content of 22%, and recover Ethanol, the ethanol extract of Dermatomella parvula and its culture medium; other steps are the same as in Example 1.

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PUM

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Abstract

The invention discloses a method for producing marasmius androsaceus fermented powder by adopting an optimized solid culture medium, belonging to the field of biological fermentation, providing rich nutrient substances for strain culture and solving the problems of long culture time and low mycelium yield. The method for producing marasmius androsaceus fermented powder by adopting the optimized solid culture medium comprises the following steps of sterilizing a solid culture medium; inoculating, culturing; extracting; concentrating and powdering, wherein the solid culture medium is prepared from the following components in parts by weight: 50-80 parts of wheat bran, 20-30 parts of rice hull, 5-20 parts of oral glucose, 0.05-0.2 part of potassium dihydrogen phosphate, 0.025-0.1 part of magnesium sulfate and the balance of water, wherein various inorganic potassium salts, especially potassium phosphate can be used as a potassium source. The substances have the stimulation action on the growth of marasins so that short culture time, high mycelium yield and shortened production time are achieved. The solid culture medium of the invention has cheap and easily-obtainable raw material components and low cost and is suitable for industrialized production.

Description

technical field [0001] The invention relates to a production method of Anluotong bacterial powder, in particular to a method for producing Anluotong bacterial powder with a solid medium. Background technique [0002] Anluotong has the functions of dredging meridians, activating collaterals, promoting blood circulation and relieving pain. It is used to treat sciatica, trigeminal neuralgia, rheumatic joint pain and other diseases. Its chemical components include mannitol, amino acids, p-hydroxycinnamic acid, and ergosterol. Studies in recent years have shown that Dermaella anluo has high medicinal and health value, and is an analgesic and antirheumatic drug that is safe for the human body and has no toxic side effects. Anluotong powder is the main component of producing Anluotong. Anluotong is obtained by culturing the bacteria of Pyrophyllum genus Anluotong of the genus P. The components of solid media currently used are wheat bran, agar, sucrose, rice hulls and water. The ...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61P19/02A61P25/02A61P29/00A01G1/04
Inventor 张秀芬
Owner 漯河市方汇药业有限公司
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