Leukocyte chemoattractant gene sequence 2 of grass carp

A chemical attraction and white blood cell technology, applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc.

Inactive Publication Date: 2010-12-08
TONGWEI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the leukocyte chemoattractin 2 ge

Method used

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  • Leukocyte chemoattractant gene sequence 2 of grass carp
  • Leukocyte chemoattractant gene sequence 2 of grass carp
  • Leukocyte chemoattractant gene sequence 2 of grass carp

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Embodiment Construction

[0021] The present invention will be further elaborated below through the following specific embodiments, but the content of the present invention is not limited thereto at all.

[0022] Extraction of total RNA

[0023] Select the experimental material—healthy female grass carp, body length 340mm, about 1.1 winter age (13 months), 1.3kg. Fresh grass carp intestinal tissues were taken out, quickly frozen in liquid nitrogen and stored in a -80°C refrigerator for later use. Total RNA was extracted by one-step method using TRIzol (Invitrogen Corporation), and the quality of total RNA was detected by formaldehyde denaturing gel electrophoresis and DNA / RNA calculator (Qenequant).

[0024] 1. Primer design basis and synthesis method

[0025] The transformant containing the EST sequence of the leukocyte chemoattractin 2 gene was screened from the grass carp intestinal cDNA library kept in our laboratory, and submitted for sequencing, and the sequencing results were compared in GenBa...

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Abstract

The invention discloses leukocyte chemoattractant genes 2 of a grass carp. By using an EST (Expressed Sequence Tag) sequence of the leukocyte chemoattractant genes 2 in a grass carp intestinal tract cDNA (complementary DNA) library saved in a laboratory as a template design primer and combining with an RACE (Rapid-Amplification of cDNA Ends) technique, the full expression sequence of the leukocyte chemoattractant genes 2 in grass carp intestinal tract cells is obtained by amplification and cloning in a PCR (Polymerase Chain Reaction) method. The invention provides important theoretical basis for the research on the structural composition of the leukocyte chemoattractant genes 2 of the grass carp, the discussion on the action and the functional zone position of the leukocyte chemoattractant genes 2 in the disease resistance of the grass carp and the difference from other species. The invention has a pioneering action on a method for exploring and establishing a link between grass carp genome planning and functional feed industrialization.

Description

technical field [0001] The invention relates to the field of gene cloning in molecular biology, in particular to the nucleotide and amino acid sequences of grass carp leukocyte chemoattractin 2 gene. Background technique [0002] LECT2 (leukocyte cell-derived chemotaxin 2) was first isolated from the culture medium of human T cell SKW-3 strain, it has neutrophil chemotactic activity, it is specifically expressed in human liver and secreted into blood. Recent studies have revealed that LECT2 may be a multifunctional protein, which is related to cell growth, differentiation, damage repair and immunity. Leukocyte chemoattractant genes have been cloned from various animals, such as bovine (Bos taurus), mouse (Mus musculus), carp (Cyprinus carpio), rainbow trout (Oncorhynchus mykiss), zebrafish (Danio rerio) and large yellow croaker ( Pseudosciaena crocea) and other species. [0003] At present, the gene of leukocyte chemoattractant 2 is still blank in the research of grass car...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/46
Inventor 徐恒袁恬府跃军顾继锐吴江
Owner TONGWEI
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