Method for restoring color of faded pathological HE section
A slicing and pathological technology, applied in the preparation of test samples, etc., can solve the problems of difficult specimens, difficult to obtain case specimens, impossible to obtain new specimens, etc., and achieve the effect of improving color.
Inactive Publication Date: 2011-11-30
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology
Moreover, due to the overall improvement of the modern medical level and the advancement of science and technology, some common diseases have been cured, and it is difficult to obtain specimens of such cases, while specimens of some rare and rare cases have been difficult or even impossible to obtain new specimens
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[0050] 1. Materials
[0051] 1.1 Screen faded teaching slides: Acute pulmonary congestion, nephritis faded slides (see figure 1 , figure 2 )
[0052] 1.2 Reagent preparation
[0053] 1.2.1Harris hematoxylin preparation:
[0054] Solution A: Hematoylin 1g
[0055] Absolute alcohol 10ml
[0056] Solution B: Potassium Aluminum Sulfate 20g
[0057] Distilled water 200ml
[0058] Fully dissolve the two solutions of A and B respectively (heat treatment is required to dissolve potassium aluminum sulfate), then mix and boil, slowly add mercuric oxide and stir to accelerate heat dissipation (note: after mercuric oxide is added, there will be bubbling phenomenon, pay attention at this time prevent the liquid from overflowing), then put it in a cold water bath to cool, return to room temperature and filter for later use. Add 4ml of glacial acetic acid per 100ml of dye solution before use.
[0059] 1.2.20.08% citric acid: Weigh 0.8g of citric acid and dissolve it in 1000ml of di...
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The invention provides a method for restoring the color of a faded pathological HE section, which comprises the following steps of: impregnating the faded pathological HE section in dimethylbenzene for 3 to 4 days, removing cover glass and label paper, continuing to impregnate the faded pathological HE section in the dimethylbenzene for one day, placing the faded pathological HE section into absolute alcohol to impregnate the faded pathological HE section for 2 hours, placing the pathological HE section into fresh absolute alcohol to impregnate the faded pathological HE section for 30 minutes, impregnating the faded pathological HE section for 10 minutes with 95 percent alcohol, washing the faded pathological HE section with tap water, impregnating the faded pathological HE section in citric acid solution for 10 to 20 minutes, taking the faded pathological HE section out, washing the faded pathological HE section with the tap water for about 20 minutes, and performing normal HE dyeing. The faded pathological HE section treated by the method of the invention is colorful and has sharp red-blue contrast.
Description
technical field [0001] The invention belongs to biological tissue staining technology, relates to pathological HE staining technology, in particular to a method for restoring color to faded pathological HE slices. Background technique [0002] Pathology teaching includes two parts: theory teaching and practice teaching, both of which complement each other in teaching and are evenly divided. In the practice teaching of pathology, pathological conventional HE slices are an important teaching tool. For the pathology teaching section, the selected cases are required to be typical, the tissue section should be complete, no wrinkles, no folds, no damage, and the sections should be brightly stained, with a sharp contrast between red and blue. play a role. However, most of the teaching slides in our department come from autopsy specimens from the 1960s and 1970s. With the passage of time, due to the age of the slices, in practice, we found that some rare and rare cases and some t...
Claims
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IPC IPC(8): G01N1/30
Inventor 瞿智玲倪娟敖启林苏铁芬
Owner HUAZHONG UNIV OF SCI & TECH