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Method for comprehensively extracting changium smyrnioide polysaccharides and amino acid

A technology for comprehensive extraction and identification of Codonopsis pilosula, applied in chemical instruments and methods, preparation of organic compounds, drug combination, etc., can solve the problems of low polysaccharide activity, small preparation amount, complicated steps, etc., and achieve less polysaccharide loss and large preparation amount , the effect of fewer processes

Inactive Publication Date: 2011-01-26
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method has the problems of complicated steps, high cost, small preparation amount and low polysaccharide activity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of Mingdangshen extract: Cut Mingdangshen root into thin slices, weigh 10g and add 120ml hot water to soak for 0.5h, heat and boil for extraction for 2h, filter, keep the filtrate, add 5 times the amount of 0.5mol / L HCl to the residue, and dissolve at 40 Extract at a constant temperature of ℃ for 1 hour, filter, add 3 times the volume of hot water to the filter residue and boil for 1 hour, filter, add 3 times the amount of 0.5mol / L HCl to the filter residue, extract at a constant temperature of 40℃ for 0.5 hours, filter, and combine the filtrates.

[0018] (2) Ion resin separation: the above-mentioned filtrate is passed through a D85 macroporous weakly acidic cation exchange resin column, first eluted with deionized water to obtain eluent A, and then eluted with 5mol / L ammonia water to obtain eluent B.

[0019] (3) Purification of polysaccharides: add eluent A to 95% ethanol, filter the precipitate, wash with acetone, and spray dry to obtain 1.03 g of the...

Embodiment 2

[0023] (1) Preparation of Mingdangshen extract: cut Mingdangshen root into thin slices, weigh 2kg and add 20L hot water to soak for 1h, heat and boil to extract for 2.5h, filter, keep the filtrate, add 6 times the amount of 0.2mol / L HCl to the residue, and dissolve at 40 Extract at a constant temperature at ℃ for 1 hour, filter, add 4 times the volume of hot water to the filter residue and boil for 1 hour, filter, add 3 times the amount of 0.2mol / L HCl to the filter residue, extract at a constant temperature of 40°C for 0.5 hours, filter, and combine the filtrates;

[0024] (2) Ion resin separation: the above-mentioned filtrate is passed through a D85 macroporous weakly acidic cation exchange resin column, first eluted with deionized water to obtain eluent A, and then eluted with 2mol / L ammonia water to obtain eluent B.

[0025] (3) Purification of polysaccharides: Add 70% ethanol to eluent A, filter the precipitate, wash with acetone, and spray dry to obtain 246 g of polysacch...

Embodiment 3

[0029] (1) Preparation of Mingdangshen extract: Cut Mingdangshen root into thin slices, weigh 5kg, add 40L hot water to soak for 40min, heat and boil for 3h, filter, keep the filtrate, add 8 times the amount of 0.4mol / L HCl to the residue, and store at 40°C Extract at a constant temperature for 1 hour, filter, add 3 times the volume of hot water to the filter residue and boil for 1 hour, filter, add 4 times the amount of 0.4mol / L HCl to the filter residue, extract at a constant temperature of 40°C for 0.5 hours, filter, and combine the filtrates;

[0030] (2) Ion resin separation: the above-mentioned filtrate is passed through a D85 macroporous weakly acidic cation exchange resin column, first eluted with deionized water to obtain eluent A, and then eluted with 1mol / L ammonia water to obtain eluent B.

[0031] (3) Purification of polysaccharides: Add 85% ethanol to eluent A, filter the precipitate, wash with acetone, and spray dry to obtain 572 g of polysaccharide samples of Co...

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PUM

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Abstract

The invention relates to a method for comprehensively extracting changium smyrnioide polysaccharides and changium smyrnioide amino acid. The method comprises the following steps of: extracting with hot water and acid water, filtering, putting the extract on a cation exchange resin column, collecting effluent liquid which is not adsorbed by resin, adding high-concentration ethanol into the effluent liquid, leaching precipitates, washing with acetone and performing freeze drying so as to obtain the changium smyrnioide polysaccharides; and eluting the resin with ammonia water, adjusting pH, separating an emulsion liquid membrane, concentrating an organic nanofiltration membrane and drying so as to obtain the changium smyrnioide amino acid. The method has the advantages of mild reaction condition, a small amount of polysaccharide loss, high amino acid activity, short process path, energy saving and suitability for mass production.

Description

Technical field: [0001] The invention belongs to the field of traditional Chinese medicines and relates to a method for comprehensively extracting polysaccharides and amino acids from Codonopsis pilosulae. Background technique: [0002] Mingdangshen is the dry root of Changium smyrnioides Wolff, a plant of Umbelliferae. It is used to "invigorate lung qi and pass downward" ("Materia Medica Congxin"); "nourishing blood and promoting body fluid, clearing away heat and detoxification" ("Materia Medica Qiuyuan"); ). Ming Codonopsis polysaccharides and amino acids are its active components, which play an important role in the efficacy of medicine. Studies have shown that Codonopsis pilosula and its polysaccharides have certain functions of strengthening the body, enhancing immunity and adapting to the original state, and have potential application value in the prevention and treatment of diseases such as cancer and infection. Ming Codonopsis can strengthen and regulate immune f...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07C227/40C07C229/00A61P37/04A61P39/00A61P11/14A61P11/06
CPCY02P20/10
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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