Method for preparing alternaria alternate culture medium

Abstract

The invention discloses a method for preparing an alternaria alternate culture medium, which comprises the following steps of: (1) preparing materials comprising 20 to 40 grams of oat, 10 to 30 milliliters of tobacco leaf juice, 1,000 milliliters of water and 10 to 25 grams of agar; and (2) preparing the culture medium, namely boiling 950 milliliters of clean water and then adding the oat into the boiling water, filtering to remove the oat by using three layers of medicinal gauze after at least 3 minutes, adding flue-cured tobacco juice into the filtrate, adding the rest clean water till 1,000 milliliters, adjusting the pH value of the culture medium to be between 5.5 and 7.5 by using lactic acid, adding agar into the culture medium, packing the culture medium, sterilizing the culture medium for at least 20 minutes at the high temperature of 121 DEG C, and cooling the culture medium. Proper tobacco leaf juice is added into the culture medium and reasonably matched with the rolled oat and the agar, and proper pH value is selected so that the growth speed of alternaria alternate hypha is increased, the spore yield is improved and the bacterial contamination rate is reduced; and the method is simple and convenient to operate and low in cost, and has good market application prospect.

Description

technical field [0001] The invention belongs to the field of indoor cultivation of plant pathogenic bacteria, and in particular relates to a preparation method of a culture medium of tobacco red spot fungus. Background technique [0002] Tobacco red spot disease is a serious disease that occurs widely in various smoking areas in the world. Tobacco red spot disease mainly begins to occur when the tobacco leaves are nearly mature. After the tobacco plants are topped, as the leaves mature, tobacco red spot disease begins to develop from the lower leaves of the tobacco plants upwards. The main damage parts are leaves, stems, pedicels and capsules are also affected. Red spot disease not only makes the tobacco leaves incomplete, but also enlarges the diseased spots during the curing process, which makes the color of the baked leaves uneven, the thickness of the leaves is uneven, and the grade declines. , Reduced reducing sugar, decreased sugar-alkaline ratio, worsened the taste,...

AI Technical Summary

Problems solved by technology

The carbon source needs organic carbon compounds, and carbohydrates are the best. Generally, glucose is the first choice as the carbon source, followed by sucrose. The carbon source can be used as a carbon frame and energy source, but too many carbon sources are easy to form low pH value, carbon Insufficient source of bacteria is prone to aging and autolysis

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