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Lignocellulose degrading bacteria and application thereof

A technology of lignocellulose and bacterial strains, applied in the direction of application, fungi, biochemical equipment and methods, etc., to improve the quality of mushroom cultivation substrates and shorten the biotransformation time

Inactive Publication Date: 2012-10-03
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been studies on the use of lignocellulosic degrading bacteria to promote the composting of agricultural waste and municipal waste, but adding lignocellulosic degrading bacteria to mushroom compost is used to increase the biotransformation rate of compost and the resulting mushroom cultivation The study on the quality of the substrate has not been reported; the screening of Penicillium strains with lignocellulose degrading ability has been reported, but the study of Penicillium expansica biological agent with high lignocellulose degrading effect has not been reported.

Method used

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  • Lignocellulose degrading bacteria and application thereof
  • Lignocellulose degrading bacteria and application thereof
  • Lignocellulose degrading bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] Embodiment The preparation of mushroom compost microbial inoculant

[0028] Fermentation tank culture solution formula is (percentage by weight): KH 2 PO 4 0.1%, NaCl 0.01%, MgSO 4 ·7H 2 O 0.03%, NaNO 3 0.25%, FeCl 3 0.001%, CaCl 2 0.01%, straw powder 0.5%, pH 7.2-7.4, prepared with water.

[0029] The W4 strain was inoculated on the PDA medium, cultured at 28-30°C for 72h, then inserted into a 500mL Erlenmeyer flask, and cultured statically at 30°C for 48h. Then put it into a 15L seed tank with 1% inoculum size, pH7.2, ventilation rate 0.5vvm, cultivate for 48 hours, then put it into a 100L fermenter with 5% inoculum size, pH7.2, ventilation rate At 0.6vvm, cultivate for 5 days.

[0030] After the fermentation is completed, the strain W4 is added to the sterilized rice bran or wheat bran according to the weight ratio of 2:10-15, sealed in aseptic bags, and stored at room temperature.

experiment example 1

[0031] Experimental Example 1 Enzyme Production Activity Experiment of Bacterial Strain W4

[0032] Liquid fermentation medium (weight percent): KH 2 PO 4 1.0g, NaCl 0.1g, MgSO 4 ·7H 2 O 0.3g, NaNO 3 2.5g, FeCl 3 0.01g, CaCl 2 0.1g, 20g straw powder, pH 7.2-7.4, prepared with water.

[0033] Preparation of cellulose crude enzyme liquid: transfer the spores of the bacterial species cultivated on PDA to normal saline, count with a hemocytometer, press 1% (10 8 spore amount) into 300ml enzyme-producing medium, 28°C, 170rmp liquid fermentation culture, sampling and measuring the enzyme activity of cellulose every 24h, the cultured liquid fermentation enzyme-producing medium was first filtered with two layers of gauze, The filtrate was then centrifuged at 5000 rpm for 10 min at 4° C., and the supernatant was taken as the prepared crude enzyme solution.

[0034] Determination of total enzyme activity: Take 0.5ml of the enzyme solution after appropriate dilution, add it t...

experiment example 2

[0042] Experimental example 2 Degradation experiment of strain W4 on straw

[0043] Straw fermentation medium: add 20g straw to 1000ml water, and add nutrient salt KH 2 PO 4 1.0g, NaCl 0.1g, MgSO 4 ·7H 2 O 0.3g, NaNO 3 2.5g, FeCl 3 0.01g, CaCl 2 0.1g, adjust the pH to 7.2-7.4, prepare with water, and sterilize under high pressure at 120°C for 20min.

[0044] Determination of straw weight loss: inoculate strain W4 into liquid seed medium to prepare bacterial liquid, inoculate 1ml of the prepared bacterial liquid into straw fermentation medium, and carry out constant temperature shaking culture. After culturing for 10 days, filter the fermentation liquid with filter paper, and remove the remaining The material was dried at 80°C and weighed, and the weight loss rate of wheat straw was calculated by weight reduction method.

[0045] Determination of straw decomposition rate: The method of Wang Yuwan et al. (1987) was used to determine the content of cellulose, hemicellu...

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Abstract

The invention discloses lignocellulose degrading bacteria and application thereof. The lignocellulose degrading bacteria is expanded penicillium W4 with the preservation number of CGMCC (China General Microbiological Culture Collection) No.4077. The stain can generate cellulose and laccase and degrade straw lignocellulose, can be used as a main effective component of microbial fungicides of a mushroom culture medium and has the effects of shortening the biological transformation time of the mushroom culture medium and improving the quality of the mushroom culture medium. Particularly for the biological transformation of a small quantity of packed mushroom culture media of a family workshop, the lignocellulose degrading bacteria has obvious advantage in environment with lower temperature.

Description

technical field [0001] The invention relates to the field of microbial inoculum, in particular to a lignocellulose-degrading microbial inoculum containing Penicillium expanses. Background technique [0002] my country is a large agricultural country. According to rough statistics, about 800 million tons of crop straw are produced every year, but the utilization rate is very low, which not only causes waste of biological resources, but also causes serious pollution to the environment. Therefore, how to make full use of these biological resources has become a constraint for our country. One of the key issues of sustainable agricultural development. [0003] Agaricus bisporus or white mushroom, its compost is generally prepared by mixing crop straw, livestock and poultry manure and a small amount of chemical fertilizer. If crop stalks can be fully utilized in mushroom production, the effective utilization rate of biological resources will be greatly improved, and the pressure o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/08C05F17/00C12R1/80
CPCY02W30/40
Inventor 范丙全王洪媛
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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